Effects of proanthocyanidins from grape seed on treatment of recurrent ulcerative colitis in rats

doi:10.1139/Y10-071

CORC > 兰州大学 > 兰州大学 > 基础医学院 > 期刊论文

	Effects of proanthocyanidins from grape seed on treatment of recurrent ulcerative colitis in rats
	Wang, YH; Yang, XL; Wang, L; Cui, MX; Cai, YQ; Li, XL; Wu, YJ; Wu, YJ (reprint author), Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China.
刊名	CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
	2010-09
卷号	88 期号:9 页码:888-898
关键词	proanthocyanidins from grape seed 2,4,6-trinitrobenzenesulfonic acid recurrent ulcerative colitis anti-inflammatory antioxidation free radicals
ISSN号	0008-4212
DOI	10.1139/Y10-071
文献子类	Article
英文摘要	The aim of the present study was to investigate the therapeutic effect and mechanism of proanthocyanidins from grape seed (GSPE) in the treatment of recurrent ulcerative colitis (UC) in rats. To induce recurrent colitis, rats were instilled with 2,4,6-trinitrobenzenesulfonic acid (TNBS) (80 mg/kg) into the colon through the cannula in the first induced phase, and then the rats were instilled a second time with TNBS (30 mg/kg) into the colon on the sixteenth day after the first induction UC. Rats were intragastrically administered GSPE (200 mg/kg) per day for 7 days after twice-induced colitis by TNBS. Sulfasalazine at 500 mg/kg was used as a positive control drug. Rats were killed 7 days after GSPE treatment. The colonic injury and inflammation were assessed by macroscopic and macroscopic damage scores, colon weight/length ratio (mg/cm), and myeloperoxidase activity. Then, superoxide dismutase, glutathione peroxidase, inducible nitric oxide synthase (iNOS) activities, and the levels of malonyldialdehyde, glutathione, and nitric oxide in serum and colonic tissues were measured. Compared with the recurrent UC group, GSPE treatment facilitated recovery of pathologic changes in the colon after induction of recurrent colitis, as demonstrated by reduced colonic weight/length ratio and macroscopic and microscopic damage scores. The myeloperoxidase and iNOS activities with malonyldialdehyde and nitric oxide levels in serum and colon tissues of colitis rats were significantly decreased in the GSPE group compared with those in the recurrent UC group. In addition, GSPE treatment was associated with notably increased superoxide dismutase, glutathione peroxidase activities, and glutathione levels of colon tissues and serum of rats. GSPE exerted a protective effect on recurrent colitis in rats by modifying the inflammatory response, inhibiting inflammatory cell infiltration and antioxidation damage, promoting damaged tissue repair to improve colonic oxidative stress, and inhibiting colonic iNOS activity to reduce the production of nitric oxide.
学科主题	Pharmacology & Pharmacy ; Physiology
出版地	OTTAWA
资助项目	国家自然科学基金项目 ; 甘肃省自然科学基金计划
项目编号	Natural Science Foundation of Gansu Province [3ZS061-A25-083] ; Natural Science Foundation of China [30672490]
语种	英语
WOS记录号	WOS:000282479300004
资助机构	NSFC ; GSSTD
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121459]
专题	基础医学院_期刊论文
通讯作者	Wu, YJ (reprint author), Lanzhou Univ, Sch Basic Med Sci, Dept Pharmacol, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Gansu, Peoples R China.
推荐引用方式 GB/T 7714	Wang, YH,Yang, XL,Wang, L,et al. Effects of proanthocyanidins from grape seed on treatment of recurrent ulcerative colitis in rats[J]. CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,2010,88(9):888-898.
APA	Wang, YH.,Yang, XL.,Wang, L.,Cui, MX.,Cai, YQ.,...&Wu, YJ .(2010).Effects of proanthocyanidins from grape seed on treatment of recurrent ulcerative colitis in rats.CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY,88(9),888-898.
MLA	Wang, YH,et al."Effects of proanthocyanidins from grape seed on treatment of recurrent ulcerative colitis in rats".CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY 88.9(2010):888-898.