Evaluation of a recombinant BCG expressing antigen Ag85B and PPE protein Rv3425 from DNA segment RD11 of Mycobacterium tuberculosis in C57BL/6 mice

doi:10.1007/s00430-008-0098-x

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	Evaluation of a recombinant BCG expressing antigen Ag85B and PPE protein Rv3425 from DNA segment RD11 of Mycobacterium tuberculosis in C57BL/6 mice
	Wang, JL; Qie, YQ; Zhu, BD; Zhang, HM; Xu, Y; Wang, QZ; Chen, JZ; Liu, W; Wang, HH; Wang, HH (reprint author), Fudan Univ, Inst Genet, Sch Life Sci, 220 Handan Rd, Shanghai 200433, Peoples R China.
刊名	MEDICAL MICROBIOLOGY AND IMMUNOLOGY
	2009-02
卷号	198 期号:1 页码:5-11
关键词	Mycobacterium tuberculosis Vaccine Ag85B Rv3425
ISSN号	0300-8584
DOI	10.1007/s00430-008-0098-x
文献子类	Article
英文摘要	Antigen 85B (Ag85B) is an important immunodominant antigen of Mycobacterium tuberculosis, and is a very promising vaccine candidate molecule. Rv3425 is a member of the subgroup 3 of the PPE family, which does not exist in all BCG strains. In this study we constructed a new rBCG which included this united gene (Ag85B-Rv3425). The level of antigen-stimulated T cells expressing IFN-gamma was significantly higher in the C57BL/6 mice vaccinated with rBCG::Ag85B-Rv3425 than with BCG. In addition, the sera from mice immunized with rBCG::Ag85B-Rv3425 revealed an increase in the specific immunoglobulin G titers than that from mice immunized with BCG. Antigen specific IgG subclass analysis showed that rBCG::Ag85B-Rv3425 tended to facilitate IgG2a production, suggesting enhancement of predominant Th1 response which in turn may facilitate increased production of protective IFN-gamma. These results suggested that this rBCG::Ag85B-Rv3425 could be a strong vaccine candidate for further study.
学科主题	Immunology ; Microbiology
出版地	NEW YORK
资助项目	国家高技术研究发展计划（863计划）
项目编号	National High Technology Research and Development Program of China [2006AA02Z445, 2006AA02Z420]
语种	英语
WOS记录号	WOS:000262576600002
资助机构	MOST
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121400]
专题	基础医学院_期刊论文
通讯作者	Wang, HH (reprint author), Fudan Univ, Inst Genet, Sch Life Sci, 220 Handan Rd, Shanghai 200433, Peoples R China.
推荐引用方式 GB/T 7714	Wang, JL,Qie, YQ,Zhu, BD,et al. Evaluation of a recombinant BCG expressing antigen Ag85B and PPE protein Rv3425 from DNA segment RD11 of Mycobacterium tuberculosis in C57BL/6 mice[J]. MEDICAL MICROBIOLOGY AND IMMUNOLOGY,2009,198(1):5-11.
APA	Wang, JL.,Qie, YQ.,Zhu, BD.,Zhang, HM.,Xu, Y.,...&Wang, HH .(2009).Evaluation of a recombinant BCG expressing antigen Ag85B and PPE protein Rv3425 from DNA segment RD11 of Mycobacterium tuberculosis in C57BL/6 mice.MEDICAL MICROBIOLOGY AND IMMUNOLOGY,198(1),5-11.
MLA	Wang, JL,et al."Evaluation of a recombinant BCG expressing antigen Ag85B and PPE protein Rv3425 from DNA segment RD11 of Mycobacterium tuberculosis in C57BL/6 mice".MEDICAL MICROBIOLOGY AND IMMUNOLOGY 198.1(2009):5-11.