Anti-osteosarcoma effects and mechanisms of 4-O-amino-phenol-4 '-demethylepipodophyllotoxin ether

doi:10.1211/jpp.60.2.0006

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	Anti-osteosarcoma effects and mechanisms of 4-O-amino-phenol-4 '-demethylepipodophyllotoxin ether
	Yang, TM; Guo, SF; Chen, CR; Zhang, XY; Li, WG; Li, WG (reprint author), Lanzhou Univ, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
刊名	JOURNAL OF PHARMACY AND PHARMACOLOGY
	2008-02
卷号	60 期号:2 页码:179-188
ISSN号	0022-3573
DOI	10.1211/jpp.60.2.0006
文献子类	Article
英文摘要	The purpose of this study was to investigate the anti-osteosarcoma effects and mechanisms of 4-O-amino-phenol-4'-demethylepipodophyllotoxin ether (ODE), a new derivative of podophyllo-toxin. The results showed that ODE inhibited proliferation of K562, OS-9901, CNE, BGC-823 and Tca-8113 cells in a time- and concentration-dependent manner as determined by microculture tetrazolium (MTT) assay. OS-9901 and K562 cells treated with ODE for 24h showed cell cycle arrest at G(2)/M and a parallel decrease in G(0)/G(1) and S phase as detected by flow cytometry (FCM). Meanwhile, a fraction of cells with hypodiploid DNA content representing apoptosis were detected by FCM. Morphology observation also revealed typical apoptotic features, including shrinkage of cellular and nuclear membranes, condensed heterochromatin around the nuclear periphery and cytoplasmic vacuolation in OS-9901 cells. Under a confocal laser scanning microscope, intracellular Ca2+ and Mg2+ concentrations were greatly increased whereas the pH value, mitochondrial membrane potential (MMP) and reactive oxygen species (ROS) were markedly reduced in OS-9901 cells after treatment with ODE. Taken together, these results suggest that the anti-osteosarcoma mechanisms of ODE are attributed to apoptosis through increasing intracellular Ca2+ and Mg2+ concentrations, and reducing pH value, MMP and ROS.
学科主题	Pharmacology & Pharmacy
出版地	LONDON
语种	英语
WOS记录号	WOS:000252855700006
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121378]
专题	基础医学院_期刊论文
通讯作者	Li, WG (reprint author), Lanzhou Univ, Key Lab Preclin Study New Drugs Gansu Prov, Lanzhou 730000, Peoples R China.
推荐引用方式 GB/T 7714	Yang, TM,Guo, SF,Chen, CR,et al. Anti-osteosarcoma effects and mechanisms of 4-O-amino-phenol-4 '-demethylepipodophyllotoxin ether[J]. JOURNAL OF PHARMACY AND PHARMACOLOGY,2008,60(2):179-188.
APA	Yang, TM,Guo, SF,Chen, CR,Zhang, XY,Li, WG,&Li, WG .(2008).Anti-osteosarcoma effects and mechanisms of 4-O-amino-phenol-4 '-demethylepipodophyllotoxin ether.JOURNAL OF PHARMACY AND PHARMACOLOGY,60(2),179-188.
MLA	Yang, TM,et al."Anti-osteosarcoma effects and mechanisms of 4-O-amino-phenol-4 '-demethylepipodophyllotoxin ether".JOURNAL OF PHARMACY AND PHARMACOLOGY 60.2(2008):179-188.