Biofunctional magnetic nanoparticles as a general agent to immobilize proteins contained in traditional Chinese medicines

doi:10.1007/s00604-006-0648-0

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	Biofunctional magnetic nanoparticles as a general agent to immobilize proteins contained in traditional Chinese medicines
	Shen, HB; Chen, W; Li, J; Li, XY; Yang, HF; Shen, HB (reprint author), Shanghai Teachers Univ, Life & Environm Sci Coll, Shanghai 200234, Peoples R China.
刊名	MICROCHIMICA ACTA
	2007-01
卷号	157 期号:1-2 页码:49-54
关键词	traditional Chinese medicines biofunctional magnetic nanoparticles protein immobilization
ISSN号	0026-3672
DOI	10.1007/s00604-006-0648-0
文献子类	Article
英文摘要	Maghemite nanoparticles were synthesized by the coprecipitation method. Silica was coated to the maghemite nanoparticles and amino silane was modified to the surface of the silica magnetic nanoparticles. We use the biofunctional magnetic nanoparticles as a general agent to immobilize and separate the proteins in a broad range from different traditional Chinese medicines. The transmission electron microscopy results showed that the average diameter of the well-dispersed silica-coated nanoparticles was about 60 nm. The Fourier transform infrared spectrum indicated that the amino group had been successfully coupled to the surface of the maghemite particles. And the protein immobilization effect was characterized by the microplate reader. The characterization results proved that the synthesized functional magnetic nanoparticles could effectively immobilize and separate the proteins from traditional Chinese medicines.
学科主题	Chemistry
出版地	WIEN
语种	英语
WOS记录号	WOS:000243325000008
内容类型	期刊论文
源URL	[http://ir.lzu.edu.cn/handle/262010/121362]
专题	基础医学院_期刊论文
通讯作者	Shen, HB (reprint author), Shanghai Teachers Univ, Life & Environm Sci Coll, Shanghai 200234, Peoples R China.
推荐引用方式 GB/T 7714	Shen, HB,Chen, W,Li, J,et al. Biofunctional magnetic nanoparticles as a general agent to immobilize proteins contained in traditional Chinese medicines[J]. MICROCHIMICA ACTA,2007,157(1-2):49-54.
APA	Shen, HB,Chen, W,Li, J,Li, XY,Yang, HF,&Shen, HB .(2007).Biofunctional magnetic nanoparticles as a general agent to immobilize proteins contained in traditional Chinese medicines.MICROCHIMICA ACTA,157(1-2),49-54.
MLA	Shen, HB,et al."Biofunctional magnetic nanoparticles as a general agent to immobilize proteins contained in traditional Chinese medicines".MICROCHIMICA ACTA 157.1-2(2007):49-54.