Structure of the Bovine A CAD8 Gene and the Association of Its Polymorphism with the Production Traits

CORC > 中国农业科学院 > 北京畜牧兽医研究所

	Structure of the Bovine A CAD8 Gene and the Association of Its Polymorphism with the Production Traits
	Hengde Li; Shangzhong Xu; Xue Gao; Hongyan Ren
刊名	Journal of Genetics and Genomics
	2007
卷号	34 期号:4 页码:315-320
关键词	ACAD8 gene structure PCR-RFLP association analysis cattle
ISSN号	1673-8527
其他题名	牛ACAD8基因结构及其多态性与生产性状的关联分析
英文摘要	Acyl-coenzyme A dehydrogenases (ACAD) are a family of nuclear-coded, mitochondrial flavoenzymes that catalyze the alpha, and beta-dehydrogenation of fatty acids. The eighth member of this family, ACAD8 catalyzes the valine catabolism. In this study, the bovine ACAD8 full-length mRNA and genomic DNA sequence were obtained and its gene structure was determined through alignment of the genomic DNA sequence to the mRNA sequence. The mRNA sequence consisted of a 1,251 bp open reading frame (ORF) flanked by a 37 bp 5′-untranslated region (UTR) and a 444 bp 3′-UTR; and its full-length genomic DNA sequence was 13,814 bp in length and included 11 exons and 10 introns. One A-G single nucleotide polymorphism (SNP) was revealed at nucleotide 13,408 (GenBank accession No. DQ435445) in the bovine ACAD8 gene by sequencing the polymerase chain reaction (PCR) products of 6 randomly selected individuals from the sample population. Different genotypes were determined by restriction fragment length polymorphism (RFLP). The association analysis of this SNP in bovine ACAD8 with production traits in 178 unrelated steers from 5 breeds showed that it had a significant effect on the daily gain and the beef tenderness (P<0.05). Cattle with the G allele grew more rapidly and the beef they produced was more tender than those with the A allele. Thus, this SNP of the bovine ACAD8 gene can be used as an indicator to improve the growth rate and the beef tenderness.
学科主题	遗传学
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/119700]
专题	北京畜牧兽医研究所
作者单位	Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100094
推荐引用方式 GB/T 7714	Hengde Li,Shangzhong Xu,Xue Gao,et al. Structure of the Bovine A CAD8 Gene and the Association of Its Polymorphism with the Production Traits[J]. Journal of Genetics and Genomics,2007,34(4):315-320.
APA	Hengde Li,Shangzhong Xu,Xue Gao,&Hongyan Ren.(2007).Structure of the Bovine A CAD8 Gene and the Association of Its Polymorphism with the Production Traits.Journal of Genetics and Genomics,34(4),315-320.
MLA	Hengde Li,et al."Structure of the Bovine A CAD8 Gene and the Association of Its Polymorphism with the Production Traits".Journal of Genetics and Genomics 34.4(2007):315-320.