Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits

CORC > 中国农业科学院 > 北京畜牧兽医研究所

	Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits
	Ma Yun 1; Xu Shangzhong 1; Gao Xue 1; Ren Hongyan 1; Xin Yaping 2; Gao Shuxin 3; Zhang Yinghan 2
刊名	遗传学报
	2006
卷号	33 期号:12 页码:1096-1104
关键词	cattle SNPs FABGL gene SSCP relationship BPI ribeye area ADG
ISSN号	0379-4172
其他题名	牛FABGL基因的克隆及其与牛生物经济学性状的相关分析
英文摘要	The complete CDS sequence of the bovine FABGL gene was determined by homology cloning approach combined with RT-PCR and 3'- and 5'-RACE. The results of sequence analysis and bioinformatics study showed that this cDNA contained 994nucleotides, with a 780 bp open reading frame (ORF) flanked by a 16 bp 5'-UTR (incompletely) and a 198 bp 3'-UTR. The deduced amino acid sequence (260 AA) shows 88% identity with the corresponding sequence in humans. Two single nucleotide substitutions,one located in intron 5 (15) at position 1 065 bp (Y = C/T) (GenBank: DQ409814) and the other in intron 8 (I8) at position 1792 bp (R = A/G), were detected using the PCR-SSCP method. Analysis of the allele frequencies of the two polymorphic sites in three different cattle breeds (Angus, Hereford, and Simmental) with different genotypes showed large differences: in locus I8, cattle with the GG genotype showed higher beef performance index (BPI) (4.283 ± 0.475 kg/cm) in comparison with cattle with the AA genotype (4.008 ± 0.465 kg/cm) (P ≤ 0.01). Regarding the ribeye area, cattle with the GG genotype showed significantly higher ribeye area (73.380 ± 13.005 cm2) compared with cattle with the AA genotype (67.744 ± 12.777 cm2) (P ≤ 0.05). In locus I5,some associations for the average daily gain (ADG) were found at the significance level of 0.01 between three different genotypes (CC, CT,TT): cattle with the TT genotype showed the highest ADG (0.652 ± 0.330 kg/d), whereas cattle with the CC genotype showed the lowest ADG value (0.421 ± 0.178 kg/d).
学科主题	遗传学
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/119617]
专题	北京畜牧兽医研究所
作者单位	1.Institue of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100094; 2.College of Animal Science and Technology, Northwest A & F University, Yangling, 712100; 3.College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao, 028000
推荐引用方式 GB/T 7714	Ma Yun,Xu Shangzhong,Gao Xue,et al. Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits[J]. 遗传学报,2006,33(12):1096-1104.
APA	Ma Yun.,Xu Shangzhong.,Gao Xue.,Ren Hongyan.,Xin Yaping.,...&Zhang Yinghan.(2006).Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits.遗传学报,33(12),1096-1104.
MLA	Ma Yun,et al."Molecular Cloning of Bovine FABGL Gene and Its Effects on Bovine Bioeconomic Traits".遗传学报 33.12(2006):1096-1104.