ISOLATION OF FIVE BIOACTIVE COMPONENTS FROM EUPATORIUM ADENOPHORUM SPRENG USING STEPWISE ELUTION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY

doi:10.1080/10826076.2011.591030

CORC > 中国农业科学院 > 农业环境与可持续发展研究所 > 环境修复研究室

	ISOLATION OF FIVE BIOACTIVE COMPONENTS FROM EUPATORIUM ADENOPHORUM SPRENG USING STEPWISE ELUTION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY
	Wei, Yun 1; Zhang, Kai 1; Zhang, Guoliang 2; Ito, Yoichiro 3
刊名	JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
	2011
卷号	34 期号:20 页码:2505-2515
关键词	eupalitin eupalitin 3-O-beta-D-galactopyranoside Eupatorium adenophorum Spreng 4 '-methyl quercetagetin 7-O-(6 ''-O-E-caffeoylglucopyranoside) quercetagetin 7-O-( 6-O-acetyl-beta-D-glucopyranoside) stepwise high-speed countercurrent chromatography
ISSN号	1082-6076
DOI	10.1080/10826076.2011.591030
通讯作者	Wei, Yun
英文摘要	Semi-preparative high-speed countercurrent chromatography (HSCCC) was successfully performed for isolation and purification of caffeic acid and 4 bioactive flavonoids from Eupatorium adenophorum Spreng using stepwise elution with a pair of two-phase solvent systems composed of ethyl acetate-methanol-water at the volume ratios of 10: 1: 10 and 5: 1: 5 (v/v). From 378.5 mg of crude extract 24.1 mg of caffeic acid, 6.7 mg of 4'-methyl quercetagetin 7-O-(6 ''-O-E-caffeoylglucopyranoside), 6.5 mg of quercetagetin 7-O-(6 ''-O-acetyl-beta-D-glucopyranoside), 31.8 mg of eupalitin 3-O-beta-D-galactopyranoside, and 36.7 mg of eupalitin were obtained with purities of 96.0%, 91.2%, 82.3%, 95.1%, and 85.6%, respectively. The structures of the separated compounds were identified by EI-MS, FAB-MS, (1)HNMR, and (13)CNMR.
学科主题	Biochemical Research Methods ; Chemistry, Analytical ; BIOCHEMICAL RESEARCH METHODS ; CHEMISTRY, ANALYTICAL
语种	英语
出版者	TAYLOR & FRANCIS INC
WOS记录号	WOS:000299376300003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19933]
专题	农业环境与可持续发展研究所_环境修复研究室
作者单位	1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China 2.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100193, Peoples R China 3.NHLBI, Bioseparat Technol Lab, Biochem & Biophys Ctr, NIH, Bethesda, MD 20892 USA
推荐引用方式 GB/T 7714	Wei, Yun,Zhang, Kai,Zhang, Guoliang,et al. ISOLATION OF FIVE BIOACTIVE COMPONENTS FROM EUPATORIUM ADENOPHORUM SPRENG USING STEPWISE ELUTION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2011,34(20):2505-2515.
APA	Wei, Yun,Zhang, Kai,Zhang, Guoliang,&Ito, Yoichiro.(2011).ISOLATION OF FIVE BIOACTIVE COMPONENTS FROM EUPATORIUM ADENOPHORUM SPRENG USING STEPWISE ELUTION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,34(20),2505-2515.
MLA	Wei, Yun,et al."ISOLATION OF FIVE BIOACTIVE COMPONENTS FROM EUPATORIUM ADENOPHORUM SPRENG USING STEPWISE ELUTION BY HIGH-SPEED COUNTERCURRENT CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 34.20(2011):2505-2515.