Isolation of Chlorogenic Acid from Flaveria bidentis (L.) Kuntze by CCC and Synthesis of Chlorogenic Acid-Intercalated Layered Double Hydroxide

doi:10.1007/s10337-010-1877-2

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	Isolation of Chlorogenic Acid from Flaveria bidentis (L.) Kuntze by CCC and Synthesis of Chlorogenic Acid-Intercalated Layered Double Hydroxide
	Wei, Yun 1; Gao, Yali 1; Xie, Qianqian 1; Zhang, Guoliang 2; Fu, Weidong 2
刊名	CHROMATOGRAPHIA
	2011
卷号	73 页码:S97-S102
关键词	Counter-current chromatography Layered double hydroxides Chlorogenic acid Flaveria bidentis (L.) Kuntze
ISSN号	0009-5893
DOI	10.1007/s10337-010-1877-2
通讯作者	Wei, Yun
英文摘要	Preparative counter-current chromatography (CCC) was successfully used for isolation and purification of chlorogenic acid from Flaveria bidentis (L.) Kuntze with a solvent system composed of ethyl acetate-methanol-water at a volume ratio of 50:1:50, v/v. Using a preparative unit of the CCC centrifuge, about 800 mg of the crude extract was separated, yielding 3.2 mg of chlorogenic acid at a purity of 92.0%. The blood pressure lowering and antivirus chlorogenic acid (C16H18O9) was intercalated into magnesium-aluminum-layered double hydroxides, which was used as host materials for drug-LDH host-guest supermolecular structures by anion exchange under a nitrogen atmosphere. Chlorogenic acid-LDH is a functional and effective drug. The product chlorogenic acid-LDH has been characterized by powder X-ray diffraction (XRD), Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA) and scanning electron micrographs (SEM). The X-ray diffraction patterns of NO3 (-) form of LDH and chlorogenic acid-LDH were compared, and the basal d spacing value of NO3 (-)-LDH layer was 8.75 (2 theta = 10.100A degrees); however, the basal reflection (003) of chlorogenic acid-LDH shifts to lower 2 theta (for 003 reflection: 2 theta A = 5.119A degrees) that is expanded to 17.25 , indicating the intercalation of chlorogenic acid into the interlayer of Mg-Al-LDH. Thermogravimetric analysis showed that chlorogenic acid stability had improved, and scanning electron micrographs showed that the morphology of the chlorogenic acid-LDH was irregular masses of distinctly thicker flakes, which was similar to the morphology of NO3 (-) form of LDH.
学科主题	Biochemical Research Methods ; Chemistry, Analytical ; BIOCHEMICAL RESEARCH METHODS ; CHEMISTRY, ANALYTICAL
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000290804900011
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19923]
专题	农业环境与可持续发展研究所_环境修复研究室
作者单位	1.Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China 2.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wei, Yun,Gao, Yali,Xie, Qianqian,et al. Isolation of Chlorogenic Acid from Flaveria bidentis (L.) Kuntze by CCC and Synthesis of Chlorogenic Acid-Intercalated Layered Double Hydroxide[J]. CHROMATOGRAPHIA,2011,73:S97-S102.
APA	Wei, Yun,Gao, Yali,Xie, Qianqian,Zhang, Guoliang,&Fu, Weidong.(2011).Isolation of Chlorogenic Acid from Flaveria bidentis (L.) Kuntze by CCC and Synthesis of Chlorogenic Acid-Intercalated Layered Double Hydroxide.CHROMATOGRAPHIA,73,S97-S102.
MLA	Wei, Yun,et al."Isolation of Chlorogenic Acid from Flaveria bidentis (L.) Kuntze by CCC and Synthesis of Chlorogenic Acid-Intercalated Layered Double Hydroxide".CHROMATOGRAPHIA 73(2011):S97-S102.