枯草芽孢杆菌B-332产抗稻瘟病菌的活性物质分析 | |
刘雪1; 田云龙1; 闫丽2; 郭萍1; 朱昌雄1 | |
刊名 | 生物技术通报 |
2012 | |
期号 | 8页码:189-193 |
关键词 | 枯草芽孢杆菌 稻瘟病菌 分离纯化 抑菌活性 组分 |
ISSN号 | 1002-5464 |
其他题名 | Separation and Purification of the Substances with Anti-Magnaporthe grisea Activity from Bacillus subtilis B-332 |
英文摘要 | 枯草芽孢杆菌B-332能够产生具有稻瘟病菌拮抗活性的物质。通过等电点法提取该抗菌液粗提物,并利用高效液相色谱-质联用仪对粗提物进行分离纯化,共得到5个组分,它们的质荷比(m/z)分别为1 045.0、1 057.8、1 072.3、1 017.5和1 031.1,是Bacillomycin D(C14-C17)的同系物(质荷比(m/z)为1 017.5的除外),它们结构间相差1个或几个-CH_2-基团;各组分均对稻瘟病菌有抑制作用,其中质荷比(m/z)为1 045.0、1 057.8、1 072.3组分的抑菌效果最强。致畸作用试验表明,这3种组分的致畸作用均为使稻瘟病菌的附着孢膨大破裂,与B-332菌株的致畸作用相同,证实了这3种组分是B-332菌株产生具有抑制稻瘟病菌作用的主要活性成分。; Bacillus subtilis B-332 could produce substances which showed antifungal activity against Magnaporthe grisea.The crude extracts of B-332 were separated by isoelectric point method,and the crude extracts were primary separated and purified using high performance liquid chromatography-mass spectrometer.The results showed that five components were achieved according to the order of the peak time,and their charge-mass ratio(m/z) were 1 045.0,1 057.8,1 072.3,1 017.5 and 1 031.1,respectively.The structures of 4 components except for the charge-mass ratio(m/z) 1 017.5 were determined as Bacillomycin D(C14-C17).The components with charge-mass ratio(m/z) 1 045.0,1 057.8,1 072.3 showed strongest inhibitory effect.Teratogenicity experiments showed that this three components has the same teratogenic effects with B-332 strain,which suggested that components with charge-mass ratio(m/z) 1 045.0,1 057.8,1 072.3 were the main active ingredient. |
学科主题 | 植物保护 |
语种 | 中文 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/17068] |
专题 | 农业环境与可持续发展研究所_环境修复研究室 |
作者单位 | 1.中国农业科学院农业环境与可持续发展研究所, 北京, 100081 2.首都博物馆, 北京, 100045 |
推荐引用方式 GB/T 7714 | 刘雪,田云龙,闫丽,等. 枯草芽孢杆菌B-332产抗稻瘟病菌的活性物质分析[J]. 生物技术通报,2012(8):189-193. |
APA | 刘雪,田云龙,闫丽,郭萍,&朱昌雄.(2012).枯草芽孢杆菌B-332产抗稻瘟病菌的活性物质分析.生物技术通报(8),189-193. |
MLA | 刘雪,et al."枯草芽孢杆菌B-332产抗稻瘟病菌的活性物质分析".生物技术通报 .8(2012):189-193. |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论