Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals

doi:10.1155/2012/764521

CORC > 中国农业科学院 > 农业环境与可持续发展研究所 > 环境工程研究室

	Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals
	Cui, Jinhui 1; Cui, Haixin 1; Wang, Yan 1; Sun, Changjiao 1; Li, Kui 2; Ren, Hongyan 2; Du, Wei 1
刊名	ADVANCES IN MATERIALS SCIENCE AND ENGINEERING
	2012
页码	-
ISSN号	1687-6822
DOI	10.1155/2012/764521
通讯作者	Cui, Jinhui
英文摘要	To evaluate the performance of the magnetic nanoparticles as gene transfer vector for breeding transgenic animals, we investigated a new approach to deliver green fluorescent protein (GFP) gene to porcine kidney 15 (PK-15) and porcine embryonic fibroblast (PEF) cells using PEI-modified magnetic nanoparticles as gene vector. The morphology of the nanoparticles and nanoparticle/DNA complexes was characterized using scanning electron microscopy. It was found that the surface of the particles becomes coarse and rough with increased average diameter, which implied the effective conjugating between nanoparticles with DNA. The zeta potential of nanoparticle/DNA complexes drops down from +29.4mV to +23.1mV comparing with pure nanoparticles. Agarose gel electrophoresis experiments show that DNA plasmids can be protected effectively against degradation of exonuclease and endonuclease. The efficiency of gene delivery was affected by the mass ratio of nanoparticle/DNA and the amount of nanoparticle/DNA complexes. We confirm that the most optimal mass ratio of nanoparticle/DNA is 1 : 1 by conducting a series of experiments. This work provides important experimental basis for the application of the magnetic nanoparticles on gene delivery to porcine somatic cells, which is significant for the achieving of breeding new transgenic cloned pigs by using somatic cell nuclear transfer technique.
学科主题	Materials Science, Multidisciplinary ; MATERIALS SCIENCE, MULTIDISCIPLINARY
语种	英语
出版者	HINDAWI PUBLISHING CORPORATION
WOS记录号	WOS:000301254400001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19902]
专题	农业环境与可持续发展研究所_环境工程研究室
作者单位	1.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Res Ctr Nanoscale Sci Agr, Beijing 100081, Peoples R China 2.Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Cui, Jinhui,Cui, Haixin,Wang, Yan,et al. Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals[J]. ADVANCES IN MATERIALS SCIENCE AND ENGINEERING,2012:-.
APA	Cui, Jinhui.,Cui, Haixin.,Wang, Yan.,Sun, Changjiao.,Li, Kui.,...&Du, Wei.(2012).Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals.ADVANCES IN MATERIALS SCIENCE AND ENGINEERING,-.
MLA	Cui, Jinhui,et al."Application of PEI-Modified Magnetic Nanoparticles as Gene Transfer Vector for the Genetic Modification of Animals".ADVANCES IN MATERIALS SCIENCE AND ENGINEERING (2012):-.