Transcriptome response of wheat Norin 10 to long-term elevated CO2 under high yield field condition
Lin Yue-bing1; Shen Cheng-guo2; Lin Er-da1; Hao Xing-yu3; Han Xue1
刊名JOURNAL OF INTEGRATIVE AGRICULTURE
2016
卷号15期号:9页码:2142-2152
关键词Norin 10 transcriptome response wheat long term elevated carbon dioxide GO enrichment
ISSN号2095-3119
DOI10.1016/S2095-3119(16)61421-1
通讯作者Lin Yue-bing
英文摘要The increasing atmospheric carbon dioxide concentration, caused by fossil fuel combustion and deforestation, plays an important role in plant growth and development. Wheat, as a major staple crop, adapts to climate change by tuning its inherent molecular mechanism, which is not well understood. The present study employed the RNA-Seq method to generate transcriptome profiles of the wheat Norin 10 in response to elevated CO2 in comparison with ambient CO2. The 10 895 787 high-quality clean reads of Norin 10 were assembled de novo using Trinity (without a reference genome) resulting in a total of 18206 candidate transcripts with significant BLAST matches. GO enrichment analysis of Norin 10 at different CO2 concentrations showed that some functional genes related to plastids, precursor metabolites, and energy, thylakoid and photosynthesis were apparently enriched at elevated CO2 (550 mu mol mol(-1)) in contrast to that at ambient CO2 (400 mu mol(-1)); these findings were further confirmed by RT-PCR analysis. The findings demonstrated the specific effects of elevated CO2 during long-term period in free air CO2 enrichment (FACE) on transcriptome response of the high yielding wheat variety, Norin 10, which has a large spike.
学科主题Agriculture, Multidisciplinary
语种英语
出版者ELSEVIER SCI LTD
WOS记录号WOS:000383411900022
内容类型期刊论文
源URL[http://111.203.20.206/handle/2HMLN22E/105454]  
专题农业环境与可持续发展研究所_气候变化研究室
作者单位1.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Key Lab Agr Environm, Minist Agr, Beijing 100081, Peoples R China
2.Source BioSci, Bioinformat Unit, Nottingham NG8 6PX, England
3.Shanxi Agr Univ, Coll Agron, Taigu 030801, Peoples R China
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GB/T 7714
Lin Yue-bing,Shen Cheng-guo,Lin Er-da,et al. Transcriptome response of wheat Norin 10 to long-term elevated CO2 under high yield field condition[J]. JOURNAL OF INTEGRATIVE AGRICULTURE,2016,15(9):2142-2152.
APA Lin Yue-bing,Shen Cheng-guo,Lin Er-da,Hao Xing-yu,&Han Xue.(2016).Transcriptome response of wheat Norin 10 to long-term elevated CO2 under high yield field condition.JOURNAL OF INTEGRATIVE AGRICULTURE,15(9),2142-2152.
MLA Lin Yue-bing,et al."Transcriptome response of wheat Norin 10 to long-term elevated CO2 under high yield field condition".JOURNAL OF INTEGRATIVE AGRICULTURE 15.9(2016):2142-2152.
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