The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia

doi:10.1016/j.cbpa.2012.09.001

	The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia
	Liu, C.1; Zhang, L. F.2; Li, N.3
刊名	COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
	2013
卷号	164 期号:4 页码:638-644
关键词	Tibetan chicken Hypoxia Embryos Globin Hemoglobin
ISSN号	1095-6433
DOI	10.1016/j.cbpa.2012.09.001
通讯作者	Liu, C.
英文摘要	Tibetan chicken (Gallus gallus) is a specific chicken breed with strong ability to resist hypoxia, especially during embryonic stage. Though this breed has lived in Tibet plateau for thousands of years, the adaptive mechanism in response to hypoxia is still unknown. In order to obtain a better understanding of the mechanism of hypoxic adaptability in high altitude, we analyzed the mRNA expression pattern of globins in the present study. The fertilized eggs from Tibetan chicken and dwarf recessive white chicken breeds were incubated under normoxic (21% O-2) and hypoxic (13% O-2) conditions, equivalent to the altitude of 3600 m. We observed that Tibetan chicken embryos had higher hatchability (48%) in hypoxia than their lowland controls (7.8%). Northern blot showed that globin mRNA expression in Tibetan chicken embryos differed greatly from lowland controls under hypoxia. The expressions of four dominant globin mRNAs, named alpha(A), alpha(D), beta(A) and beta(H), were significantly induced under hypoxia. Tibetan chicken embryos had lower globin mRNA level in red cells than that of lowland controls at day 19 (P<0.05). Based on real-time PCR the same result was confirmed. Furthermore, we observed accumulation of globins induced by hypoxia in red cells by performing the separation of globin analysis, showing higher level of globins in red cells of Tibetan chicken embryos than that of lowland chicken embryos. Overall, our results provide new evidence that flexible regulation of globins at the level of transcription and translation may play a role in allowing the Tibetan chicken embryo to resist hypoxia. (C) 2012 Published by Elsevier Inc.
学科主题	Biochemistry & Molecular Biology ; Physiology ; Zoology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; PHYSIOLOGY ; ZOOLOGY
语种	英语
出版者	ELSEVIER SCIENCE INC
WOS记录号	WOS:000316829200013
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/19857]
专题	农业环境与可持续发展研究所
作者单位	1.Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China 2.Zhejiang Univ, Coll Anim Sci, Hangzhou 310029, Zhejiang, Peoples R China 3.China Agr Univ, State Key Lab Agrobiotechnol, Beijing 10094, Peoples R China
推荐引用方式 GB/T 7714	Liu, C.,Zhang, L. F.,Li, N.. The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,2013,164(4):638-644.
APA	Liu, C.,Zhang, L. F.,&Li, N..(2013).The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY,164(4),638-644.
MLA	Liu, C.,et al."The specific expression pattern of globin mRNAs in Tibetan chicken during late embryonic stage under hypoxia".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY 164.4(2013):638-644.