昆虫病原真菌抗紫外保护剂的筛选和评价

	昆虫病原真菌抗紫外保护剂的筛选和评价
	农向群 1; 张泽华 1; 金正俊 2
刊名	植物保护学报
	2005
卷号	32 期号:4 页码:402-406
关键词	紫外保护剂昆虫病原真菌分生孢子保护
ISSN号	0577-7518
其他题名	Screening and evaluation of ultraviolet protectants for entomopathogenic fungi in laboratory
英文摘要	在室内检测了17种紫外保护剂和4种组合对3种昆虫病原真菌即球孢白僵菌Beauveria bassiana、金龟子绿僵菌Metarhizium anisopliae、蜡蚧轮枝孢Verticillium lacani分生孢子的保护作用.结果表明,苯并三唑类2#的紫外保护作用最好,用紫外杀菌灯照射30 min,苯并三唑类2#对3种菌株孢子的保护效率均达90%以上;苯并三唑类3#、苯甲酮类2#、荧光黄钠、刚果红的保护效率可达60%.组合使用紫外保护剂并不能增强保护效果.选用苯并三唑类2#时,适宜的溶剂是花生油加正己烷(1:1,v/v),使用浓度应大于0.75%.用254、312、365 nm波长紫外灯照射40 min内,1%苯并三唑类2#对3种菌株孢子的保护效率可保持90%,照射180 min后,保护效率在56%～77%。; The UV-screen effect of 17 UV-protectants and 4 combinations for conidia of Beauveria bassi-ana, Metarhizium anisopliae and Verticillium lacanii was inspected in laboratory. UVP-2 ( benzotriazole 2 ) was confirmed to be the most effective protectant. Protective efficiency of UVP-2 to spores of entomopathogenic fungi was over 90% , and other 4 UVPs (benzotriazole 3 , oxybenzone 2 , sodium uranine and Congo red) were about 60% after 30 min exposure of spores to UV. Mixture of UVPs did not show higher effectiveness compared to single UVP. The mixture of peanut oil and n-hexane was suitable solute for UVP-2. The effective concentration of UVP-2 should be higher than 0. 75% for the protection of fungal conidia. After 40 min and 180 min exposure of fungal conidia to UV light (at X254r,,., > Vii2nm or \vs5nm) > the protective efficiency of UVP-2 could be increased over 90% and 56% -77% , respectively.
学科主题	植物保护
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17517]
专题	农业环境与可持续发展研究所
作者单位	1.中国农业科学院农业环境与可持续发展研究所, 北京, 100081 2.韩国农村振兴厅国家农业科学与技术研究院, 韩国
推荐引用方式 GB/T 7714	农向群,张泽华,金正俊. 昆虫病原真菌抗紫外保护剂的筛选和评价[J]. 植物保护学报,2005,32(4):402-406.
APA	农向群,张泽华,&金正俊.(2005).昆虫病原真菌抗紫外保护剂的筛选和评价.植物保护学报,32(4),402-406.
MLA	农向群,et al."昆虫病原真菌抗紫外保护剂的筛选和评价".植物保护学报 32.4(2005):402-406.