青贮玉米-牛粪尿体系的~(15)N标记及氮素转化研究

	青贮玉米-牛粪尿体系的~(15)N标记及氮素转化研究
	岳现录 1; 廖上强 2; 冀宏杰 2; 张维理 2; 左余宝 2; 荣向农 2
刊名	中国生态农业学报
	2012
卷号	20 期号:1 页码:24-27
关键词	~(15)N标记硫酸铵牛粪尿青贮玉米氮素回收氮素转化
ISSN号	1671-3990
其他题名	Nitrogen-15 labeling and nitrogen transformation in silage maize-cattle manure system
英文摘要	~(15)N示踪技术已开始应用于畜禽粪便氮素循环与利用研究领域,而~(15)N在畜禽粪便不同组分和不同形态氮素中的丰度与数量将直接影响到畜禽粪便~(15)N示踪去向与氮素实际去向的一致性。为了解~(15)N在畜禽粪便标记过程的转化特点和在标记粪尿的分布特征,本文首先采用改进的、含有~(15)N标记硫酸铵(60 atom%~(15)N)的Hoagland营养液砂培种植~(15)N玉米,然后将~(15)N玉米和普通玉米以55∶45的氮配比作为混合青贮饲料饲喂1头已空腹2 d的2龄黄牛,饲喂4 d后停喂2 d,收集全部牛粪尿并对其不同组分和形态氮素的~(15)N丰度和数量进行分析。结果表明:标记玉米、混合青贮饲料、牛粪尿的~(15)N丰度分别为48.024%、26.579%和8.044%;标记玉米对硫酸铵~(15)N的回收率为26.3%,牛粪尿对标记玉米~(15)N回收率为36.0%。在收集的牛粪尿氮中,牛粪全氮、牛尿全氮、牛粪铵态氮和牛尿铵态氮量分别占70.25%、29.75%、5.44%和0.03%,其~(15)N丰度分别为9.223%、5.261%、6.505%和5.419%。在短期内通过饲喂黄牛~(15)N青贮饲料制备的标记牛粪尿中,~(15)N丰度在不同组分和形态氮素中的分布并不相同,牛尿氮的~(15)N丰度低于牛粪氮,矿质态和易于矿化态氮的~(15)N丰度低于不易矿化态氮。; ~(15)N tracer technique has been used in N cycling and utilization for livestock manure.The abundance and amount of ~(15)N in different manure components and N forms affect whether ~(15)N fate is consistent with all manure N.The purpose of this article is to better understand the characteristics of ~(15)N transformation in silage maize-cattle manure system and distribution in cattle feces and urine by using ~(15)N tracer technique.First,a sand culturing experiment was conducted by watering improved Hoagland nutrient solution with ~(15)N labeled ammonium sulfate(AS,60 atom% ~(15)N) to cultivate ~(15)N labeled maize("Nongda-108").Then,~(15)N labeled maize was mixed with unlabeled maize at a ratio of 55︰45,according to their amount of nitrogen,to get mixed ~(15)N forage.Finally,a 2-years-old cattle starved for 2 days was fed on the mixed ~(15)N forage for 4 days and starved 2 days again.During the 6 days,cattle feces and urine were collected and measured separately.The results showed that ~(15)N abundance was 48.024% in labeled maize,26.579% in mixed silages,and 8.044% in cattle manure.In addition,26.3% of AS ~(15)N was discovered in maize,36.0% of silage ~(15)N was discovered in cattle manure.In all the manure N collected,fecal N,urine N,fecal ammonium N and urine ammonium N accounted for 70.25%,29.75%,5.44% and 0.03%,their ~(15)N abundance were 9.223%,5.261%,6.505% and 5.419%,respectively.In ~(15)N labeled manure of cattle fed on ~(15)N labeled silage in a short period,the abundance of ~(15)N in cattle urine and feces,and in different N forms were different.The ~(15)N abundance of urine was lower than that of feces,and ~(15)N abundance of mineral and easy-mineralized nitrogen was lower than that of hardly mineralized nitrogen.
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/17112]
专题	农业环境与可持续发展研究所
作者单位	1.中国农业科学院农业环境与可持续发展研究所, 农业部作物营养与施肥重点开放实验室, 北京, 100081 2.中国农业科学院农业资源与农业区划研究所, 农业部作物营养与施肥重点开放实验室, 北京, 100081
推荐引用方式 GB/T 7714	岳现录,廖上强,冀宏杰,等. 青贮玉米-牛粪尿体系的~(15)N标记及氮素转化研究[J]. 中国生态农业学报,2012,20(1):24-27.
APA	岳现录,廖上强,冀宏杰,张维理,左余宝,&荣向农.(2012).青贮玉米-牛粪尿体系的~(15)N标记及氮素转化研究.中国生态农业学报,20(1),24-27.
MLA	岳现录,et al."青贮玉米-牛粪尿体系的~(15)N标记及氮素转化研究".中国生态农业学报 20.1(2012):24-27.