Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage

doi:10.1007/s11240-012-0131-z

	Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage
	Yuan, Su-xia; Su, Yan-bin; Liu, Yu-mei; Fang, Zhi-yuan; Yang, Li-mei; Zhuang, Mu; Zhang, Yang-yong; Sun, Pei-tian
刊名	PLANT CELL TISSUE AND ORGAN CULTURE
	2012
卷号	110 期号:1 页码:69-76
关键词	White cabbage (Brassica oleracea L. var. capitata) Microspore culture Embryogenesis pH 2-(N-Morpholino) ethanesulfonic acid Arabinogalactan-protein
ISSN号	0167-6857
DOI	10.1007/s11240-012-0131-z
通讯作者	Yuan, Su-xia
英文摘要	The objective of this study was to improve induction of embryogenesis in white cabbage (Brassica oleracea var. capitata) microspore cultures. The effect of NLN-13 liquid medium pH on isolated microspore embryogenesis was investigated in five white cabbage genotypes. Relatively high pH (6.2 or 6.4) was more effective on microspore embryogenesis in most of the white cabbage genotypes than the pH of 5.8, especially for inducing microspore-derived embryos in recalcitrant genotype 'Zhonggan No. 8'. Based on this, 2-(N-Morpholino) ethanesulfonic acid (MES) and the arabinogalactan-protein from gum arabic were tested on four out of five genotypes to see if they could increase embryo yield in microspore cultures. Adding MES or gum arabic alone was effective for these four genotypes, but the frequency of embryos derived from microspores was still low. However, the combination of 10 mg l(-1) gum arabic and 3 mM MES in NLN-13 at pH 6.4 significantly enhanced microspore embryogenesis efficiency (with embryo production of 4.57-222.97 embryos per bud), especially with recalcitrant genotype "Zhonggan No. 8" for which it was increased by about 35-fold.
学科主题	Biotechnology & Applied Microbiology ; Plant Sciences ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000304664300008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101096]
专题	蔬菜花卉研究所_花卉研究室
作者单位	Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Yuan, Su-xia,Su, Yan-bin,Liu, Yu-mei,et al. Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage[J]. PLANT CELL TISSUE AND ORGAN CULTURE,2012,110(1):69-76.
APA	Yuan, Su-xia.,Su, Yan-bin.,Liu, Yu-mei.,Fang, Zhi-yuan.,Yang, Li-mei.,...&Sun, Pei-tian.(2012).Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage.PLANT CELL TISSUE AND ORGAN CULTURE,110(1),69-76.
MLA	Yuan, Su-xia,et al."Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage".PLANT CELL TISSUE AND ORGAN CULTURE 110.1(2012):69-76.