茄子甲硫氨酸亚砜还原酶（SmMsrA）基因cDNA全长的克隆和分析

	茄子甲硫氨酸亚砜还原酶（SmMsrA）基因cDNA全长的克隆和分析
	张映 1; 陈钰辉 2; 张振贤 1; 方智远 1; 连勇 2; 刘富中 2
刊名	园艺学报
	2011
卷号	38 期号:8 页码:1469-1478
关键词	茄子单性结实甲硫氨酸亚砜还原酶表达分析
ISSN号	0513-353X
其他题名	cDNA Cloning and Characterization of a Eggplant Peptide Methionine Sulfoxide Reductase（SmMsrA）
英文摘要	以茄子单性结实品系D-10花后7 d的单性结实果实为试材,并以在茄子单性结实抑制差减文库中差异表达的EST片段Z569为基础,利用RACE技术,获得一个934 bp的cDNA全长序列,为甲硫氨酸亚砜还原酶A基因,命名为SmMsrA。基因编码区共600 bp,编码199个氨基酸。氨基酸序列分析显示该基因编码的蛋白具有甲硫氨酸亚砜还原酶基因家族结构域和保守基本序列GCFWG,与杨树甲硫氨酸亚砜还原酶A（MsrA）蛋白三级结构相似性较高,为67%。蛋白多序列比对和进化树分析表明,SmMsrA与番茄MsrA蛋白的同源性最高（92%）,进化距离最近。采用荧光定量PCR对单性结实品系D-10和非单性结实品系03-2中不同结实性果实发育过程中SmMsrA的表达量进行测定,结果表明:在不同结实性的子房和果实发育过程中SmMsrA基因都有表达,单性结实品系在低温条件下开花当天子房中的SmMsrA的表达量最高。; Using parthenocarpic fruit of eggplant（Solanum melongena）harvested seven days after anthesis from line D-10 as material,based on the EST Z569 from eggplant parthenocarpic suppression subtractive hybridization library,a full cDNA（934 bp）was cloned by rapid amplification of cDNA ends （RACE）.The cloned gene was Methionine sulfoxide reductaseA（MsrA）gene（SmMsrA）,containing an open reading frame（600 bp）and encoding a protein of 199 amino acids.The predicted protein was a member of the peptide methionine sulfoxide reductase（PMSR）superfamily,and contained a conserved sequence GCFWG.The tertiary structure of SmMsrA was similar to the poplar MsrA model（2j89）with a similarity of 67%.Multiple sequence alignments and phylogenetic tree analyses showed that SmMsrA had the highest similarit（y92%）with the tomato MsrA（accession number P54153）.The expression of SmMsrA was determined by real-time quantitative RT-PCR during the fruit development of line D-10 （parthenocarpic fruits and seeded fruits）and line 03-2（unparthenocarpic fruits and seeded fruits）.The result showed that the SmMsrA gene was expressed throughout eggplant fruit development in different cultivars,and in parthenocarpic ovaries the highest expression was at anthesis day and under suboptimal temperatures.
学科主题	园艺
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100627]
专题	蔬菜花卉研究所_茄科研究室
作者单位	1.中国农业大学农学与生物技术学院, 北京, 100193 2.中国农业科学院蔬菜花卉研究所, 北京, 100081
推荐引用方式 GB/T 7714	张映,陈钰辉,张振贤,等. 茄子甲硫氨酸亚砜还原酶（SmMsrA）基因cDNA全长的克隆和分析[J]. 园艺学报,2011,38(8):1469-1478.
APA	张映,陈钰辉,张振贤,方智远,连勇,&刘富中.(2011).茄子甲硫氨酸亚砜还原酶（SmMsrA）基因cDNA全长的克隆和分析.园艺学报,38(8),1469-1478.
MLA	张映,et al."茄子甲硫氨酸亚砜还原酶（SmMsrA）基因cDNA全长的克隆和分析".园艺学报 38.8(2011):1469-1478.