Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens | |
Wu, Jian1,2; Zhao, Fang-Jie3; Ghandilyan, Artak1; Logoteta, Barbara3; Guzman, Myriam Olortegui1; Schat, Henk4; Wang, Xiaowu2; Aarts, Mark G. M.1 | |
刊名 | PLANT AND SOIL
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2009 | |
卷号 | 325期号:1-2页码:79-95 |
关键词 | TcZNT5 TcZNT6 Thlaspi caerulescens Hyperaccumulation Metal transporter Zinc Cadmium |
ISSN号 | 0032-079X |
DOI | 10.1007/s11104-009-0151-6 |
通讯作者 | Wu, Jian |
英文摘要 | The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes. |
学科主题 | Agronomy ; Plant Sciences ; Soil Science ; AGRONOMY ; PLANT SCIENCES ; SOIL SCIENCE |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000272383900008 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/101199] ![]() |
专题 | 蔬菜花卉研究所_生物技术研究室 |
作者单位 | 1.Wageningen Univ, Genet Lab, NL-6700 AH Wageningen, Netherlands 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Rothamsted Res, Harpenden AL5 2JQ, Herts, England 4.Vrije Univ Amsterdam, Fac Biol, NL-1081 HV Amsterdam, Netherlands |
推荐引用方式 GB/T 7714 | Wu, Jian,Zhao, Fang-Jie,Ghandilyan, Artak,et al. Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens[J]. PLANT AND SOIL,2009,325(1-2):79-95. |
APA | Wu, Jian.,Zhao, Fang-Jie.,Ghandilyan, Artak.,Logoteta, Barbara.,Guzman, Myriam Olortegui.,...&Aarts, Mark G. M..(2009).Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens.PLANT AND SOIL,325(1-2),79-95. |
MLA | Wu, Jian,et al."Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens".PLANT AND SOIL 325.1-2(2009):79-95. |
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