The LmSNF1 Gene Is Required for Pathogenicity in the Canola Blackleg Pathogen Leptosphaeria maculans

doi:10.1371/journal.pone.0092503

	The LmSNF1 Gene Is Required for Pathogenicity in the Canola Blackleg Pathogen Leptosphaeria maculans
	Feng, Jie 1; Zhang, Hui 2; Strelkov, Stephen E.3; Hwang, Sheau-Fang 1
刊名	PLOS ONE
	2014
卷号	9 期号:3 页码:-
ISSN号	1932-6203
DOI	10.1371/journal.pone.0092503
通讯作者	Feng, Jie
英文摘要	Leptosphaeria maculans is a fungal pathogen causing blackleg in canola. Its virulence has been attributed, among other factors, to the activity of hydrolytic cell wall degrading enzymes (CWDEs). Studies on the pathogenicity function of CWDEs in plant pathogenic fungi have been difficult due to gene redundancy. In microorganisms many CWDE genes are repressed by glucose and derepressed by the function of the sucrose non-fermenting protein kinase 1 gene (SNF1). To address the molecular function of SNF1 in L. maculans, the ortholog of SNF1 (LmSNF1) was cloned and functionally characterized using a gene knockout strategy. Growth of the LmSNF1 knockout strains was severely disrupted, as was sporulation, spore germination and the ability to attach on the plant surface. When inoculated on canola cotyledons, the LmSNF1 knockout strains could not cause any symptoms, indicating the loss of pathogenicity. The expression of 11 selected CWDE genes and a pathogenicity gene (LopB) was significantly down-regulated in the LmSNF1 knockout strains. In conclusion, knockout of LmSNF1 prevents L. maculans from properly derepressing the production of CWDEs, compromises the utilization of certain carbon sources, and impairs fungal pathogenicity on canola.
学科主题	Multidisciplinary Sciences ; MULTIDISCIPLINARY SCIENCES
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
WOS记录号	WOS:000333254100151
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100986]
专题	蔬菜花卉研究所_十字花科研究室
作者单位	1.Alberta Agr & Rural Dev, Crop Diversificat Ctr North, Edmonton, AB, Canada 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100193, Peoples R China 3.Univ Alberta, Dept Agr Food & Nutr Sci, Edmonton, AB, Canada
推荐引用方式 GB/T 7714	Feng, Jie,Zhang, Hui,Strelkov, Stephen E.,et al. The LmSNF1 Gene Is Required for Pathogenicity in the Canola Blackleg Pathogen Leptosphaeria maculans[J]. PLOS ONE,2014,9(3):-.
APA	Feng, Jie,Zhang, Hui,Strelkov, Stephen E.,&Hwang, Sheau-Fang.(2014).The LmSNF1 Gene Is Required for Pathogenicity in the Canola Blackleg Pathogen Leptosphaeria maculans.PLOS ONE,9(3),-.
MLA	Feng, Jie,et al."The LmSNF1 Gene Is Required for Pathogenicity in the Canola Blackleg Pathogen Leptosphaeria maculans".PLOS ONE 9.3(2014):-.