Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings

doi:10.1016/j.plaphy.2016.07.012

	Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings
	Zhang, Xue 1; Yu, Hong Jun 1; Zhang, Xiao Meng 1; Yang, Xue Yong 1; Zhao, Wen Chao 3; Li, Qiang 1; Jiang, Wei Jie 1,2
刊名	PLANT PHYSIOLOGY AND BIOCHEMISTRY
	2016
卷号	108 页码:222-230
关键词	Nitrogen deficiency Cucumber L-ascorbic acid Ascorbate biosynthesis pathways Ascorbate recycling pathway
ISSN号	0981-9428
DOI	10.1016/j.plaphy.2016.07.012
通讯作者	Zhang, Xue
英文摘要	L-Ascorbic acid (AsA, ascorbate) is one of the most abundant natural antioxidants, and it is an important factor in the nutritional quality of cucumber. In this work, key enzymes involved in the ascorbic acid biosynthesis and recycling pathway in cucumber seedlings under nitrogen deficiency were investigated at the levels of transcription and enzyme activity. The activities of myo-inositol oxygenase (MIOX) and transcript levels of MIOXs increased dramatically, while the activities of ascorbate oxidase (AO) and glutathione reductase (GR) and transcript levels of AOs and GR2 decreased significantly in N-limited leaves, as did the ascorbate concentration, in nitrogen-deficient cucumber seedlings. The activities of other enzymes and transcript levels of other genes involved in the ascorbate recycling pathway and ascorbate synthesis pathways decreased or remained unchanged under nitrogen deficiency. These results indicate that nitrogen deficiency induced genes involved in the ascorbate-glutathione recycling and myoinositol pathway in cucumber leaves. Thus, the AO, GR and MIOX involved in the pathways might play roles in AsA accumulation. (C) 2016 Elsevier Masson SAS. All rights reserved.
学科主题	Plant Sciences
语种	英语
出版者	ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
WOS记录号	WOS:000386989700024
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105898]
专题	蔬菜花卉研究所_栽培与采后技术研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Key Lab Hort Crops Genet Improvement, Minist Agr, Beijing 100081, Peoples R China 2.Xinjiang Agr Univ, Urumqi 830052, Xinjiang, Peoples R China 3.Beijing Agr Univ, Plant Sci & Technol Coll, Beijing Key Lab Agr Applicat & New Technol, Beijing 102206, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Xue,Yu, Hong Jun,Zhang, Xiao Meng,et al. Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings[J]. PLANT PHYSIOLOGY AND BIOCHEMISTRY,2016,108:222-230.
APA	Zhang, Xue.,Yu, Hong Jun.,Zhang, Xiao Meng.,Yang, Xue Yong.,Zhao, Wen Chao.,...&Jiang, Wei Jie.(2016).Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings.PLANT PHYSIOLOGY AND BIOCHEMISTRY,108,222-230.
MLA	Zhang, Xue,et al."Effect of nitrogen deficiency on ascorbic acid biosynthesis and recycling pathway in cucumber seedlings".PLANT PHYSIOLOGY AND BIOCHEMISTRY 108(2016):222-230.