Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)

doi:10.1603/0022-0493-101.6.1888

	Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)
	Zhou, Xiao-Mao 1,2; Wu, Qing-Jun 1; Zhang, You-Jun 2; Bai, Lian-Yang 2; Huang, Xiong-Ying 2
刊名	JOURNAL OF ECONOMIC ENTOMOLOGY
	2008
卷号	101 期号:6 页码:1888-1896
关键词	Plutella xylostella GABA receptor functional expression Xenopus oocyte abamectin
ISSN号	0022-0493
DOI	10.1603/0022-0493-101.6.1888
通讯作者	Zhou, Xiao-Mao
英文摘要	A full-length cDNA, with an open reading frame (ORF) of 1,449 bp, encoding a subunit of the gamma-aminobutyric acid (GABA)-activated chloride channel was isolated from Plutella xylostella (L.) (Lepidoptera: Plutellidae) (GenBank accession no. EF156251). The subunit gene encoded a 483-amino acid polypeptide that showed 84% sequence identity with DmRdl subunit (U02042) (Drosophila melanogaster resistant to dieldrin). When expressed in Xenopus laevis oocytes, the subunit assembled as a functional homomeric complex activated by GABA and abamectin in a dose-dependent manner. The EC(50) value of GABA was 0.49 mM (0.41-0.58) (n = 5). However, the responses to abamectin were very robust, with an EC(50) of 4.85 mu M (4.02-5.89) (n = 6), indicating that abamectin wits >100-fold more potent in activating chloride currents than GABA. The results suggest that this subunit is vital to the formation of a functional channel and contains the binding site of abamectin.
学科主题	Entomology ; ENTOMOLOGY
语种	英语
出版者	ENTOMOLOGICAL SOC AMER
WOS记录号	WOS:000261430700024
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101225]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 2.Hunan Agr Univ, Dept Plant Protect, Changsha 410128, Hunan, Peoples R China
推荐引用方式 GB/T 7714	Zhou, Xiao-Mao,Wu, Qing-Jun,Zhang, You-Jun,et al. Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)[J]. JOURNAL OF ECONOMIC ENTOMOLOGY,2008,101(6):1888-1896.
APA	Zhou, Xiao-Mao,Wu, Qing-Jun,Zhang, You-Jun,Bai, Lian-Yang,&Huang, Xiong-Ying.(2008).Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae).JOURNAL OF ECONOMIC ENTOMOLOGY,101(6),1888-1896.
MLA	Zhou, Xiao-Mao,et al."Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)".JOURNAL OF ECONOMIC ENTOMOLOGY 101.6(2008):1888-1896.