Determination of a novel paclitaxel derivative (NPD-103) in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry

doi:10.1002/bmc.1146

	Determination of a novel paclitaxel derivative (NPD-103) in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry
	Zhang, Shuang-Qing 1; Chen, Guo-Hua 2
刊名	BIOMEDICAL CHROMATOGRAPHY
	2009
卷号	23 期号:5 页码:510-515
关键词	novel paclitaxel derivative (NPD-103) ultra-performance liquid chromatography-tandem mass spectrometry human plasma
ISSN号	0269-3879
DOI	10.1002/bmc.1146
通讯作者	Zhang, Shuang-Qing
英文摘要	A sensitive and specific ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS-MS) method for quantification of a newly developed anticancer agent NPD-103 has been established. An aliquot of human plasma sample (200 mu L) was spiked with (13)C-labeled paclitaxel (internal standard) and extracted with 1.3 mL of tert-butyl methyl ether. NPD-103 was quantitated on a C(18) column with methanol-0.1% formic acid (75:25, v/v) as mobile phase using UPLC-MS-MS operating in positive electrospray ionization mode with a total run time of 3.0 min. For NPD-103 at the concentrations of 1.0, 5.0 and 10.0 mu g/mL in human plasma, the absolute extraction recoveries were 95.58, 102.43 and 97.77%, respectively. The linear quantification range of the method was 0.1-20.0 mu g/mL in human plasma with linear correlation coefficients greater than 0.999. The intra- and inter-day accuracy for NPD-103 at 1.0, 5.0 and 10.0 mu g/mL levels in human plasma fell into the ranges of 95.29-100.00% and 91.04-94.21%, and the intra- and inter-day precisions were in the ranges of 8.96-11.79% and 7.25-10.63%, respectively. This assay is applied to determination of half-life of NPD-103 in human plasma. Copyright (C) 2008 John Wiley & Sons, Ltd.
学科主题	Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy ; BIOCHEMICAL RESEARCH METHODS ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; CHEMISTRY, ANALYTICAL ; PHARMACOLOGY & PHARMACY
语种	英语
出版者	JOHN WILEY & SONS LTD
WOS记录号	WOS:000265435800009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101212]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Univ Mississippi, Sch Pharm, Dept Pharmaceut, University, MS 38677 USA 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Shuang-Qing,Chen, Guo-Hua. Determination of a novel paclitaxel derivative (NPD-103) in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry[J]. BIOMEDICAL CHROMATOGRAPHY,2009,23(5):510-515.
APA	Zhang, Shuang-Qing,&Chen, Guo-Hua.(2009).Determination of a novel paclitaxel derivative (NPD-103) in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry.BIOMEDICAL CHROMATOGRAPHY,23(5),510-515.
MLA	Zhang, Shuang-Qing,et al."Determination of a novel paclitaxel derivative (NPD-103) in human plasma by ultra-performance liquid chromatography-tandem mass spectrometry".BIOMEDICAL CHROMATOGRAPHY 23.5(2009):510-515.