The alpha 6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad

doi:10.1016/j.pestbp.2014.03.004

	The alpha 6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad
	Hou, Wenjie 1; Liu, Qiulei 1; Tian, Lixia 1; Wu, Qingjun 1; Zhang, Youjun 1; Xie, Wen 1; Wang, Shaoli 1; San Miguel, Keri 2; Funderburk, Joe 3; Scott, Jeffrey G.2
刊名	PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
	2014
卷号	111 页码:60-67
关键词	Western flower thrips Spinosyns Mechanism of resistance Inheritance Nicotinic acetylcholine receptor
ISSN号	0048-3575
DOI	10.1016/j.pestbp.2014.03.004
通讯作者	Hou, Wenjie
英文摘要	Insects evolve resistance which constrains the sustainable use of insecticides. Spinosyns, a class of environmentally-friendly macrolide insecticides, is not an exception. The mode of inheritance and the mechanisms of resistance to spinosad (the most common spinosyn insecticide) in Frankliniella occidentalis (Western flower thrips, WFT) were investigated in this study. Resistance (170,000-fold) was autosomal and completely recessive. Recent studies showed that deletion of the nicotinic acetylcholine receptor alpha 6 subunit gene resulted in strains of Drosophila melanogaster, Plutella xylostella and Bactrocera dorsalis that are resistant to spinosad, indicating that nAChR alpha 6 subunit maybe important for the toxic action of this insecticide. Conversely, a G275E mutation of this subunit in F. occidentalis was recently proposed as the mechanism of resistance to spinosad. We cloned and characterized nAChR alpha 6 from three susceptible and two spinosad resistant strains from China and the USA. The Fo alpha 6 cDNA is 1873 bp and the open reading frame is 1458 bp which encodes 485 amino acid residues with a predicted molecular weight of 53.5-kDa, the 5' and 3' UTRs are 121 and 294 bp, respectively. There was no difference in the cDNA sequence between the resistant and susceptible thrips, suggesting the G275E mutation does not confer resistance in these populations. Ten isoforms of Foa6, arising from alternative splicing, were isolated and did not differ between the spinosad-susceptible and resistant strains. Quantitative real time PCR analysis showed Fo alpha 6 was highly expressed in the first instar larva, pupa and adult, and the expression levels were 3.67, 2.47, 1.38 times that of the second instar larva. The expression level was not significantly different between the susceptible and resistant strains. These results indicate that Foa6 is not involved in resistance to spinosad in F. occidentalis from China and the USA. (C) 2014 Elsevier Inc. All rights reserved.
学科主题	Biochemistry & Molecular Biology ; Entomology ; Physiology ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; ENTOMOLOGY ; PHYSIOLOGY
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000336888800010
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100981]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Cornell Univ, Dept Entomol, Ithaca, NY 14853 USA 3.Univ Florida, Dept Entomol & Nematol, Quincy, FL 32351 USA
推荐引用方式 GB/T 7714	Hou, Wenjie,Liu, Qiulei,Tian, Lixia,et al. The alpha 6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad[J]. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,2014,111:60-67.
APA	Hou, Wenjie.,Liu, Qiulei.,Tian, Lixia.,Wu, Qingjun.,Zhang, Youjun.,...&Scott, Jeffrey G..(2014).The alpha 6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad.PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY,111,60-67.
MLA	Hou, Wenjie,et al."The alpha 6 nicotinic acetylcholine receptor subunit of Frankliniella occidentalis is not involved in resistance to spinosad".PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY 111(2014):60-67.