Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy

doi:10.3964/j.issn.1000-0593(2015)05-1243-05

	Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy
	Wang Wei-ping 1,2; Chai A-li 2; Shi Yan-xia 2; Xie Xue-wen 2; Li Bao-ju 2
刊名	SPECTROSCOPY AND SPECTRAL ANALYSIS
	2015
卷号	35 期号:5 页码:1243-1247
关键词	Fourier transform infrared spectroscopy FTIR Chinese cabbage clubroot Plasmodiophora brassicae Model
ISSN号	1000-0593
DOI	10.3964/j.issn.1000-0593(2015)05-1243-05
通讯作者	Wang Wei-ping
英文摘要	Clubroot, caused by Plasmodiophora brassicae, is considered the most devastating soilborne disease in Brassica crops. It has emerged as a serious disease threatening the cruciferous crop production industry in China. Nowadays, the detection techniques for P. brassicae are laborious, time-consuming and low sensitivity. Rapid and effective detection methods are needed. The objective of this study is to develop a Fourier transform infrared spectrometer (FTIR) technique for detection of P. brassicae effectively and accurately. FTIR and Real-time PCR techniques were applied in quantitative detection of P. brassicae. Chinese cabbages were inoculated with P. brassicae. By analyzing the FTIR spectra of P. brassicae, infected clubroots and healthy roots, three specific bands 1 105, 1 145 and 1 228 cm(-1) were selected. According to the correlation between the peak areas at these sensitive bands and Real-time PCR Ct value, quantitative evaluation model of P. brassicae was established based on FTIR y=34. 17+12. 24x(1)-9. 81x(2)-6. 05x(3), r=0. 98 (p<0. 05). To validate accuracy of the model, 10 clubroot samples were selected randomly from field, and detected by FTIR spectrum model, the results showed that the average error is 1. 60%. This demonstrated that the FTIR technology is an available one for the quantitative detection of P. brassicae in clubroot, and it provides a new method for quantitative and quickly detection of Chinese cabbage clubroot.
学科主题	Spectroscopy
语种	中文
出版者	OFFICE SPECTROSCOPY & SPECTRAL ANALYSIS
WOS记录号	WOS:000354142900019
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100903]
专题	蔬菜花卉研究所_植物保护研究室
作者单位	1.Shenyang Agr Univ, Coll Plant Protect, Shenyang 100866, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang Wei-ping,Chai A-li,Shi Yan-xia,et al. Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy[J]. SPECTROSCOPY AND SPECTRAL ANALYSIS,2015,35(5):1243-1247.
APA	Wang Wei-ping,Chai A-li,Shi Yan-xia,Xie Xue-wen,&Li Bao-ju.(2015).Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy.SPECTROSCOPY AND SPECTRAL ANALYSIS,35(5),1243-1247.
MLA	Wang Wei-ping,et al."Quantitative Detection of Chinese Cabbage Clubroot Based on FTIR Spectroscopy".SPECTROSCOPY AND SPECTRAL ANALYSIS 35.5(2015):1243-1247.