Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)

doi:10.3390/ijms17071034

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	Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)
	Shi, Caihua 1; Yang, Fengshan 2; Zhu, Xun 3; Du, Erxia 4; Yang, Yuting 1; Wang, Shaoli 3; Wu, Qingjun 3; Zhang, Youjun 3
刊名	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
	2016
卷号	17 期号:7 页码:-
关键词	Bradysia odoriphaga normalization reference genes RefFinder
ISSN号	1422-0067
DOI	10.3390/ijms17071034
通讯作者	Shi, Caihua
英文摘要	The soil insect Bradysiaodoriphaga (Diptera: Sciaridae) causes substantial damage to Chinese chive. Suitable reference genes in B. odoriphaga (Bradysiaodoriphaga) have yet to be identified for normalizing target gene expression among samples by quantitative real-time PCR (qRT-PCR). This study was focused on identifying the expression stability of 12 candidate housekeeping genes in B. odoriphaga under various experiment conditions. The final stability ranking of 12 housekeeping genes was obtained with RefFinder, and the most suitable number of reference genes was analyzed by GeNorm. The results revealed that the most appropriate sets of internal controls were RPS15, RPL18, and RPS18 across developmental phases; RPS15, RPL28, and GAPDH across temperatures; RPS15 and RPL18 across pesticide treatments; RSP5, RPS18, and SDHA across photoperiods; ACTb, RPS18, and RPS15 across diets; RPS13 and RPL28 across populations; and RPS15, ACTb, and RPS18 across all samples. The use of the most suitable reference genes versus an arbitrarily selected reference gene resulted in significant differences in the analysis of a target gene expression. HSP23 in B. odoriphaga was found to be up-regulated under low temperatures. These results will contribute to the standardization of qRT-PCR and will also be valuable for further research on gene function in B. odoriphaga.
学科主题	Chemistry, Multidisciplinary
语种	英语
出版者	MDPI AG
WOS记录号	WOS:000381500900049
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105919]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Yangtze Univ, Coll Agr, Jingzhou 434025, Peoples R China 2.Heilongjiang Univ, Coll Life Sci, Harbin 150080, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 4.Univ Connecticut, Ctr Hlth, Dept Med, Farmington, CT 06030 USA
推荐引用方式 GB/T 7714	Shi, Caihua,Yang, Fengshan,Zhu, Xun,et al. Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2016,17(7):-.
APA	Shi, Caihua.,Yang, Fengshan.,Zhu, Xun.,Du, Erxia.,Yang, Yuting.,...&Zhang, Youjun.(2016).Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae).INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,17(7),-.
MLA	Shi, Caihua,et al."Evaluation of Housekeeping Genes for Quantitative Real-Time PCR Analysis of Bradysia odoriphaga (Diptera: Sciaridae)".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 17.7(2016):-.