A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time | |
Yuan, Yu-Xiang1,2; Wu, Jian1; Sun, Ri-Fei1; Zhang, Xiao-Wei2; Xu, Dong-Hui1; Bonnema, Guusje3; Wang, Xiao-Wu1 | |
刊名 | JOURNAL OF EXPERIMENTAL BOTANY
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2009 | |
卷号 | 60期号:4页码:1299-1308 |
关键词 | BrFLC1 flowering time splicing pattern splicing site mutation |
ISSN号 | 0022-0957 |
DOI | 10.1093/jxb/erp010 |
通讯作者 | Yuan, Yu-Xiang |
英文摘要 | FLOWERING LOCUS C (FLC), encoding a MADS-domain transcription factor in Arabidopsis, is a repressor of flowering involved in the vernalization pathway. This provides a good reference for Brassica species. Genomes of Brassica species contain several FLC homologues and several of these colocalize with flowering-time QTL. Here the analysis of sequence variation of BrFLC1 in Brassica rapa and its association with the flowering-time phenotype is reported. The analysis revealed that a G -> A polymorphism at the 5' splice site in intron 6 of BrFLC1 is associated with flowering phenotype. Three BrFLC1 alleles with alternative splicing patterns, including two with different parts of intron 6 retained and one with the entire exon 6 excluded from the transcript, were identified in addition to alleles with normal splicing. It was inferred that aberrant splicing of the pre-mRNA leads to loss-of-function of BrFLC1. A CAPS marker was developed for this locus to distinguish Pi6+1(G) and Pi6+1(A). The polymorphism detected with this marker was significantly associated with flowering time in a collection of 121 B. rapa accessions and in a segregating Chinese cabbage doubled-haploid population. These findings suggest that a naturally occurring splicing mutation in the BrFLC1 gene contributes greatly to flowering-time variation in B. rapa. |
学科主题 | Plant Sciences ; PLANT SCIENCES |
语种 | 英语 |
出版者 | OXFORD UNIV PRESS |
WOS记录号 | WOS:000264390600023 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/101214] ![]() |
专题 | 蔬菜花卉研究所_职能部门 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China 3.Univ Wageningen & Res Ctr, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands |
推荐引用方式 GB/T 7714 | Yuan, Yu-Xiang,Wu, Jian,Sun, Ri-Fei,et al. A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time[J]. JOURNAL OF EXPERIMENTAL BOTANY,2009,60(4):1299-1308. |
APA | Yuan, Yu-Xiang.,Wu, Jian.,Sun, Ri-Fei.,Zhang, Xiao-Wei.,Xu, Dong-Hui.,...&Wang, Xiao-Wu.(2009).A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time.JOURNAL OF EXPERIMENTAL BOTANY,60(4),1299-1308. |
MLA | Yuan, Yu-Xiang,et al."A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time".JOURNAL OF EXPERIMENTAL BOTANY 60.4(2009):1299-1308. |
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