A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time

doi:10.1093/jxb/erp010

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	A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time
	Yuan, Yu-Xiang 1,2; Wu, Jian 1; Sun, Ri-Fei 1; Zhang, Xiao-Wei 2; Xu, Dong-Hui 1; Bonnema, Guusje 3; Wang, Xiao-Wu 1
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2009
卷号	60 期号:4 页码:1299-1308
关键词	BrFLC1 flowering time splicing pattern splicing site mutation
ISSN号	0022-0957
DOI	10.1093/jxb/erp010
通讯作者	Yuan, Yu-Xiang
英文摘要	FLOWERING LOCUS C (FLC), encoding a MADS-domain transcription factor in Arabidopsis, is a repressor of flowering involved in the vernalization pathway. This provides a good reference for Brassica species. Genomes of Brassica species contain several FLC homologues and several of these colocalize with flowering-time QTL. Here the analysis of sequence variation of BrFLC1 in Brassica rapa and its association with the flowering-time phenotype is reported. The analysis revealed that a G -> A polymorphism at the 5' splice site in intron 6 of BrFLC1 is associated with flowering phenotype. Three BrFLC1 alleles with alternative splicing patterns, including two with different parts of intron 6 retained and one with the entire exon 6 excluded from the transcript, were identified in addition to alleles with normal splicing. It was inferred that aberrant splicing of the pre-mRNA leads to loss-of-function of BrFLC1. A CAPS marker was developed for this locus to distinguish Pi6+1(G) and Pi6+1(A). The polymorphism detected with this marker was significantly associated with flowering time in a collection of 121 B. rapa accessions and in a segregating Chinese cabbage doubled-haploid population. These findings suggest that a naturally occurring splicing mutation in the BrFLC1 gene contributes greatly to flowering-time variation in B. rapa.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000264390600023
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101214]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 2.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China 3.Univ Wageningen & Res Ctr, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Yuan, Yu-Xiang,Wu, Jian,Sun, Ri-Fei,et al. A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time[J]. JOURNAL OF EXPERIMENTAL BOTANY,2009,60(4):1299-1308.
APA	Yuan, Yu-Xiang.,Wu, Jian.,Sun, Ri-Fei.,Zhang, Xiao-Wei.,Xu, Dong-Hui.,...&Wang, Xiao-Wu.(2009).A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time.JOURNAL OF EXPERIMENTAL BOTANY,60(4),1299-1308.
MLA	Yuan, Yu-Xiang,et al."A naturally occurring splicing site mutation in the Brassica rapa FLC1 gene is associated with variation in flowering time".JOURNAL OF EXPERIMENTAL BOTANY 60.4(2009):1299-1308.