Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development

doi:10.1093/jxb/ers375

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	Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development
	Wang, Yong-Qiang 1; Yang, Yong 2; Fei, Zhangjun 2,3; Yuan, Hui 1; Fish, Tara 2; Thannhauser, Theodore W.2; Mazourek, Michael 1; Kochian, Leon V.2; Wang, Xiaowu 4; Li, Li 1,2
刊名	JOURNAL OF EXPERIMENTAL BOTANY
	2013
卷号	64 期号:4 页码:949-961
关键词	Carrot cauliflower chromoplast papaya pepper proteomics tomato watermelon
ISSN号	0022-0957
DOI	10.1093/jxb/ers375
通讯作者	Wang, Yong-Qiang
英文摘要	Chromoplasts are unique plastids that accumulate massive amounts of carotenoids. To gain a general and comparative characterization of chromoplast proteins, this study performed proteomic analysis of chromoplasts from six carotenoid-rich crops: watermelon, tomato, carrot, orange cauliflower, red papaya, and red bell pepper. Stromal and membrane proteins of chromoplasts were separated by 1D gel electrophoresis and analysed using nLC-MS/MS. A total of 9532262 proteins from chromoplasts of different crop species were identified. Approximately 60% of the identified proteins were predicted to be plastid localized. Functional classification using MapMan bins revealed large numbers of proteins involved in protein metabolism, transport, amino acid metabolism, lipid metabolism, and redox in chromoplasts from all six species. Seventeen core carotenoid metabolic enzymes were identified. Phytoene synthase, phytoene desaturase, ?-carotene desaturase, 9-cis-epoxycarotenoid dioxygenase, and carotenoid cleavage dioxygenase 1 were found in almost all crops, suggesting relative abundance of them among the carotenoid pathway enzymes. Chromoplasts from different crops contained abundant amounts of ATP synthase and adenine nucleotide translocator, which indicates an important role of ATP production and transport in chromoplast development. Distinctive abundant proteins were observed in chromoplast from different crops, including capsanthin/capsorubin synthase and fibrillins in pepper, superoxide dismutase in watermelon, carrot, and cauliflower, and glutathione-S-transferease in papaya. The comparative analysis of chromoplast proteins among six crop species offers new insights into the general metabolism and function of chromoplasts as well as the uniqueness of chromoplasts in specific crop species. This work provides reference datasets for future experimental study of chromoplast biogenesis, development, and regulation in plants.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	OXFORD UNIV PRESS
WOS记录号	WOS:000316003600012
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101070]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Cornell Univ, Dept Plant Breeding & Genet, Ithaca, NY 14853 USA 2.Cornell Univ, USDA ARS, Robert W Holley Ctr Agr & Hlth, Ithaca, NY 14853 USA 3.Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA 4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Yong-Qiang,Yang, Yong,Fei, Zhangjun,et al. Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development[J]. JOURNAL OF EXPERIMENTAL BOTANY,2013,64(4):949-961.
APA	Wang, Yong-Qiang.,Yang, Yong.,Fei, Zhangjun.,Yuan, Hui.,Fish, Tara.,...&Li, Li.(2013).Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development.JOURNAL OF EXPERIMENTAL BOTANY,64(4),949-961.
MLA	Wang, Yong-Qiang,et al."Proteomic analysis of chromoplasts from six crop species reveals insights into chromoplast function and development".JOURNAL OF EXPERIMENTAL BOTANY 64.4(2013):949-961.