Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

doi:10.1016/j.gene.2016.01.005

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	Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties
	Liu, Yating 1,2; Xie, Wen 2; Yang, Xin 2; Guo, Litao 2; Wang, Shaoli 2; Wu, Qingjun 2; Yang, Zezhong 2; Zhou, Xuguo 3; Zhang, Youjun 2
刊名	GENE
	2016
卷号	580 期号:2 页码:104-110
关键词	PSI Haplodiploidy Bemisia tabaci Sex determination Alternative splicing
ISSN号	0378-1119
DOI	10.1016/j.gene.2016.01.005
通讯作者	Liu, Yating
英文摘要	The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino adds. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect. (C) 2016 Elsevier B.V. All rights reserved.
学科主题	Genetics & Heredity
语种	英语
出版者	ELSEVIER SCIENCE BV
WOS记录号	WOS:000370885100003
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100827]
专题	蔬菜花卉研究所_职能部门
作者单位	1.Hunan Agr Univ, Coll Plant Protect, Changsha 410128, Hunan, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 3.Univ Kentucky, Dept Entomol, Lexington, KY 40546 USA
推荐引用方式 GB/T 7714	Liu, Yating,Xie, Wen,Yang, Xin,et al. Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties[J]. GENE,2016,580(2):104-110.
APA	Liu, Yating.,Xie, Wen.,Yang, Xin.,Guo, Litao.,Wang, Shaoli.,...&Zhang, Youjun.(2016).Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties.GENE,580(2),104-110.
MLA	Liu, Yating,et al."Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties".GENE 580.2(2016):104-110.