yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing

doi:10.1007/s12041-016-0724-0

CORC > 中国农业科学院 > 蔬菜花卉研究所

	yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing
	Feng, Yalan 1,2,3,4; Zhao, Yongying 2,3,4,5; Wang, Ketao 2,6,7; Li, Yong Chun 2,3,4; Wang, Xiang 2,3,4; Yin, Jun 2,3,4
刊名	JOURNAL OF GENETICS
	2016
卷号	95 期号:4 页码:957-964
关键词	ribonucleic acid-sequencing vernalization cultivar Jing841 cultivar Liaochun10
ISSN号	0022-1333
DOI	10.1007/s12041-016-0724-0
通讯作者	Feng, Yalan
英文摘要	This study aimed to identify vernalization responsive genes in the winter wheat cultivar Jing841 by comparing the transcriptome data with that of a spring wheat cultivar Liaochun10. For each cultivar, seedlings before and after the vernalization treatment were sequenced by Solexa/Illumina sequencing. Genes differentially expressed after and before vernalization were identified as differentially expressed genes (DEGs) using false discovery rate (FDR) <= 0.001 and \| log2 (fold change)\| > 1 as cutoffs. The Jing841-specific DEGs were screened and subjected to functional annotation using gene ontology (GO) database. Vernalization responsive genes among the specific genes were selected for validation by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and the expression change over the time was investigated for the top 11 genes with the most significant expression differences. A total of 138,062 unigenes were obtained. Overall, 636 DEGs were identified as vernalization responsive genes including some known genes such as VRN-1 and COR14a, and some unknown contigs. The qRT-PCR validated changes in the expression of 18 DEGs that were detected by RNA-seq. Among them, 11 genes displayed four different types of expression patterns over time during the 30-day-long vernalization treatment. Genes or contigs such as VRN-A1, COR14a, IRIP, unigene1806 and Cl18953. Contig2 probably have critical roles in vernalization.
学科主题	Genetics & Heredity
语种	英语
出版者	INDIAN ACAD SCIENCES
WOS记录号	WOS:000390260600023
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/105900]
专题	蔬菜花卉研究所
作者单位	1.Henan Univ Sci & Technol, Coll Agr, Luoyang 471003, Peoples R China 2.Henan Agr Univ, Natl Engn Res Ctr Wheat, Zhengzhou 450002, Peoples R China 3.Henan Agr Univ, Collaborat Innovat Ctr Henan Grain Crops, Zhengzhou 450002, Peoples R China 4.Henan Agr Univ, Natl Key Lab Wheat & Maize Crop Sci, Zhengzhou 450002, Peoples R China 5.Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou 450002, Peoples R China 6.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Sino Dutch Joint Lab Hort Genom, Minist Agr,Key Lab Biol & Genet Improvement Hort, Beijing 100081, Peoples R China 7.China Agr Univ, Beijing 100083, Peoples R China
推荐引用方式 GB/T 7714	Feng, Yalan,Zhao, Yongying,Wang, Ketao,et al. yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing[J]. JOURNAL OF GENETICS,2016,95(4):957-964.
APA	Feng, Yalan,Zhao, Yongying,Wang, Ketao,Li, Yong Chun,Wang, Xiang,&Yin, Jun.(2016).yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing.JOURNAL OF GENETICS,95(4),957-964.
MLA	Feng, Yalan,et al."yy Identification of vernalization responsive genes in the winter wheat cultivar Jing841 by transcriptome sequencing".JOURNAL OF GENETICS 95.4(2016):957-964.