Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae) | |
Zhou, Xiao-Mao1,2; Wu, Qing-Jun1; Zhang, You-Jun2; Bai, Lian-Yang2; Huang, Xiong-Ying2 | |
刊名 | JOURNAL OF ECONOMIC ENTOMOLOGY
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2008 | |
卷号 | 101期号:6页码:1888-1896 |
关键词 | Plutella xylostella GABA receptor functional expression Xenopus oocyte abamectin |
ISSN号 | 0022-0493 |
DOI | 10.1603/0022-0493-101.6.1888 |
通讯作者 | Zhou, Xiao-Mao |
英文摘要 | A full-length cDNA, with an open reading frame (ORF) of 1,449 bp, encoding a subunit of the gamma-aminobutyric acid (GABA)-activated chloride channel was isolated from Plutella xylostella (L.) (Lepidoptera: Plutellidae) (GenBank accession no. EF156251). The subunit gene encoded a 483-amino acid polypeptide that showed 84% sequence identity with DmRdl subunit (U02042) (Drosophila melanogaster resistant to dieldrin). When expressed in Xenopus laevis oocytes, the subunit assembled as a functional homomeric complex activated by GABA and abamectin in a dose-dependent manner. The EC(50) value of GABA was 0.49 mM (0.41-0.58) (n = 5). However, the responses to abamectin were very robust, with an EC(50) of 4.85 mu M (4.02-5.89) (n = 6), indicating that abamectin wits >100-fold more potent in activating chloride currents than GABA. The results suggest that this subunit is vital to the formation of a functional channel and contains the binding site of abamectin. |
学科主题 | Entomology ; ENTOMOLOGY |
语种 | 英语 |
出版者 | ENTOMOLOGICAL SOC AMER |
WOS记录号 | WOS:000261430700024 |
内容类型 | 期刊论文 |
源URL | [http://111.203.20.206/handle/2HMLN22E/101997] ![]() |
专题 | 蔬菜花卉研究所 |
作者单位 | 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Dept Plant Protect, Beijing 100081, Peoples R China 2.Hunan Agr Univ, Dept Plant Protect, Changsha 410128, Hunan, Peoples R China |
推荐引用方式 GB/T 7714 | Zhou, Xiao-Mao,Wu, Qing-Jun,Zhang, You-Jun,et al. Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)[J]. JOURNAL OF ECONOMIC ENTOMOLOGY,2008,101(6):1888-1896. |
APA | Zhou, Xiao-Mao,Wu, Qing-Jun,Zhang, You-Jun,Bai, Lian-Yang,&Huang, Xiong-Ying.(2008).Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae).JOURNAL OF ECONOMIC ENTOMOLOGY,101(6),1888-1896. |
MLA | Zhou, Xiao-Mao,et al."Cloning and Characterization of a GABA Receptor from Plutella xylostella (Lepidoptera: Plutellidae)".JOURNAL OF ECONOMIC ENTOMOLOGY 101.6(2008):1888-1896. |
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