Sequencing the Potato Genome: Outline and First Results to Come from the Elucidation of the Sequence of the World's Third Most Important Food Crop

doi:10.1007/s12230-009-9097-8

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	Sequencing the Potato Genome: Outline and First Results to Come from the Elucidation of the Sequence of the World's Third Most Important Food Crop
	Visser, Richard G. F.1; Bachem, Christian W. B.1; de Boer, Jan M.1; Bryan, Glenn J.2; Chakrabati, Swarup K.3; Feingold, Sergio 4; Gromadka, Robert 5; van Ham, Roeland C. H. J.6; Huang, Sanwen 7; Jacobs, Jeanne M. E.8
刊名	AMERICAN JOURNAL OF POTATO RESEARCH
	2009
卷号	86 期号:6 页码:417-429
关键词	Potato genome sequencing consortium Cytogenetics Genetics Next generation sequencing AFLP
ISSN号	1099-209X
DOI	10.1007/s12230-009-9097-8
通讯作者	Visser, Richard G. F.
英文摘要	Potato is a member of the Solanaceae, a plant family that includes several other economically important species, such as tomato, eggplant, petunia, tobacco and pepper. The Potato Genome Sequencing Consortium (PGSC) aims to elucidate the complete genome sequence of potato, the third most important food crop in the world. The PGSC is a collaboration between 13 research groups from China, India, Poland, Russia, the Netherlands, Ireland, Argentina, Brazil, Chile, Peru, USA, New Zealand and the UK. The potato genome consists of 12 chromosomes and has a (haploid) length of approximately 840 million base pairs, making it a medium-sized plant genome. The sequencing project builds on a diploid potato genomic bacterial artificial chromosome (BAC) clone library of 78000 clones, which has been fingerprinted and aligned into similar to 7000 physical map contigs. In addition, the BAC-ends have been sequenced and are publicly available. Approximately 30000 BACs are anchored to the Ultra High Density genetic map of potato, composed of 10000 unique AFLP (TM) markers. From this integrated genetic-physical map, between 50 to 150 seed BACs have currently been identified for every chromosome. Fluorescent in situ hybridization experiments on selected BAC clones confirm these anchor points. The seed clones provide the starting point for a BAC-by-BAC sequencing strategy. This strategy is being complemented by whole genome shotgun sequencing approaches using both 454 GS FLX and Illumina GA2 instruments. Assembly and annotation of the sequence data will be performed using publicly available and tailor-made tools. The availability of the annotated data will help to characterize germplasm collections based on allelic variance and to assist potato breeders to more fully exploit the genetic potential of potato.
学科主题	Agronomy ; AGRONOMY
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000276131500001
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101984]
专题	蔬菜花卉研究所
作者单位	1.Univ Wageningen & Res Ctr, Wageningen UR Plant Breeding, NL-6700 AJ Wageningen, Netherlands 2.SCRI, Genet Programme, Dundee DD2 5DA, Scotland 3.Cent Potato Res Inst, Shimla 171001, Himachal Prades, India 4.INTA, EEA Balcarce, Lab Agrobiotecnol, Balcarce, Argentina 5.Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland 6.WUR, Plant Res Int Ctr BioSyst Genom, NL-6708 PB Wageningen, Netherlands 7.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 8.New Zealand Inst Plant & Food Res Ltd, Christchurch, New Zealand 9.RAS, Ctr Bioengn, Moscow 117901, Russia 10.EMBRAPA, BR-359970 Brasilia, DF, Brazil
推荐引用方式 GB/T 7714	Visser, Richard G. F.,Bachem, Christian W. B.,de Boer, Jan M.,et al. Sequencing the Potato Genome: Outline and First Results to Come from the Elucidation of the Sequence of the World's Third Most Important Food Crop[J]. AMERICAN JOURNAL OF POTATO RESEARCH,2009,86(6):417-429.
APA	Visser, Richard G. F..,Bachem, Christian W. B..,de Boer, Jan M..,Bryan, Glenn J..,Chakrabati, Swarup K..,...&Tang, Xiaomin.(2009).Sequencing the Potato Genome: Outline and First Results to Come from the Elucidation of the Sequence of the World's Third Most Important Food Crop.AMERICAN JOURNAL OF POTATO RESEARCH,86(6),417-429.
MLA	Visser, Richard G. F.,et al."Sequencing the Potato Genome: Outline and First Results to Come from the Elucidation of the Sequence of the World's Third Most Important Food Crop".AMERICAN JOURNAL OF POTATO RESEARCH 86.6(2009):417-429.