Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens

doi:10.1007/s11104-009-0151-6

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens
	Wu, Jian 1,2; Zhao, Fang-Jie 3; Ghandilyan, Artak 1; Logoteta, Barbara 3; Guzman, Myriam Olortegui 1; Schat, Henk 4; Wang, Xiaowu 2; Aarts, Mark G. M.1
刊名	PLANT AND SOIL
	2009
卷号	325 期号:2017-01-02 页码:79-95
关键词	TcZNT5 TcZNT6 Thlaspi caerulescens Hyperaccumulation Metal transporter Zinc Cadmium
ISSN号	0032-079X
DOI	10.1007/s11104-009-0151-6
通讯作者	Wu, Jian
英文摘要	The heavy metal hyperaccumulator Thlaspi caerulescens expresses several ZIP-like genes at higher levels than their orthologues in non-hyperaccumulator species, but it is not clear why. To elucidate the function of the T. caerulescens orthologues of the Arabidopsis thaliana ZIP5 and ZIP6 genes, full-length cDNAs of TcZNT5-LC and TcZNT6-LC were cloned, their expression was examined and genes were expressed in A. thaliana. Transcript level analysis revealed the constitutively high expression of these two genes in T. caerulescens compared to AtZIP5 and AtZIP6 genes and differential expression of both genes when comparing two accessions of T. caerulescens with different metal accumulation properties. Expression of TcZNT5-LC in A. thaliana did not modify Cd or Zn tolerance, but mildly affected the root and shoot Zn concentrations towards a hyperaccumulator shoot to root concentration ratio. A. thaliana zip5 knock-out mutants showed increased tolerance to Cd and decreased seed mineral concentrations. Expression of TcZNT6-LC enhanced the Cd sensitivity of A. thaliana, but no phenotype was observed for the zip6 mutant. In conclusion, the changes in expression of TcZNT5-LC and TcZNT6-LC upon changes in Zn or Cd exposure indicate both genes act in metal homeostasis, but their CaMV 35S-mediated expression in A. thaliana does not create T. caerulescens hyperaccumulator phenotypes.
学科主题	Agronomy ; Plant Sciences ; Soil Science ; AGRONOMY ; PLANT SCIENCES ; SOIL SCIENCE
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000272383900008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101983]
专题	蔬菜花卉研究所
作者单位	1.Wageningen Univ, Genet Lab, NL-6700 AH Wageningen, Netherlands 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Rothamsted Res, Harpenden AL5 2JQ, Herts, England 4.Vrije Univ Amsterdam, Fac Biol, NL-1081 HV Amsterdam, Netherlands
推荐引用方式 GB/T 7714	Wu, Jian,Zhao, Fang-Jie,Ghandilyan, Artak,et al. Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens[J]. PLANT AND SOIL,2009,325(2017-01-02):79-95.
APA	Wu, Jian.,Zhao, Fang-Jie.,Ghandilyan, Artak.,Logoteta, Barbara.,Guzman, Myriam Olortegui.,...&Aarts, Mark G. M..(2009).Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens.PLANT AND SOIL,325(2017-01-02),79-95.
MLA	Wu, Jian,et al."Identification and functional analysis of two ZIP metal transporters of the hyperaccumulator Thlaspi caerulescens".PLANT AND SOIL 325.2017-01-02(2009):79-95.