Overexpression in tobacco of a tomato GMPase gene improves tolerance to both low and high temperature stress by enhancing antioxidation capacity

doi:10.1007/s00299-011-1009-y

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Overexpression in tobacco of a tomato GMPase gene improves tolerance to both low and high temperature stress by enhancing antioxidation capacity
	Wang, Hua-Sen 1; Yu, Chao 1; Zhu, Zhu-Jun 1; Yu, Xian-Chang 2
刊名	PLANT CELL REPORTS
	2011
卷号	30 期号:6 页码:1029-1040
关键词	GDP-D-mannose pyrophosphorylase L-Ascorbic acid Reactive oxygen species Temperature stresses
ISSN号	0721-7714
DOI	10.1007/s00299-011-1009-y
通讯作者	Wang, Hua-Sen
英文摘要	GDP-mannose pyrophosphorylase (GMPase: EC 2.7.7.22) plays a crucial role in the synthesis of l-ascorbate (AsA) and the consequent detoxification of reactive oxygen species (ROS). Herein, a GMPase (accession ID DQ449030) was identified and cloned from tomato. The full-length cDNA sequence of this gene contains 1,498 bp nucleotides encoding a putative protein with 361 amino acid residues of approximate molecular weight 43 kDa. Northern blot analysis revealed that the GMPase was expressed in all examined tomato tissues, but its expression level was up-regulated in tomato plants subjected to abnormal temperatures. We then overexpressed this tomato GMPase in tobacco plants and observed that the activity of GMPase and the content of AsA were significantly increased by two- to fourfold in the leaves of transgenic tobacco plants. The effect of this gene overexpression was superimposed by the treatments of high or low temperature in tobacco, since the activities of both chloroplastic SOD (superoxide dismutase EC 1.15.1.1), APX (ascorbate peroxidase EC 1.11.1.7) and the content of AsA in leaves were significantly higher in transgenic plants than those of WT, while the contents of H2O2 and O-2 (-center dot) were reduced. Meanwhile, relative electric conductivity increased less in transgenic plants than that in WT, and the net photosynthetic rate (P (n)) and the maximal photochemical efficiency of PSII (F (v)/F (m)) of transgenic plants were notably higher than those of WT under temperature stresses. In conclusion, the overexpression of GMPase increased the content of AsA, thereby leading to the increase in tolerance to temperature stress in transgenic plants.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER
WOS记录号	WOS:000290542800008
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101958]
专题	蔬菜花卉研究所
作者单位	1.Zhejiang A&F Univ, Dept Hort, Sch Agr & Food Sci, Linan 311300, Zhejiang, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 10081, Peoples R China
推荐引用方式 GB/T 7714	Wang, Hua-Sen,Yu, Chao,Zhu, Zhu-Jun,et al. Overexpression in tobacco of a tomato GMPase gene improves tolerance to both low and high temperature stress by enhancing antioxidation capacity[J]. PLANT CELL REPORTS,2011,30(6):1029-1040.
APA	Wang, Hua-Sen,Yu, Chao,Zhu, Zhu-Jun,&Yu, Xian-Chang.(2011).Overexpression in tobacco of a tomato GMPase gene improves tolerance to both low and high temperature stress by enhancing antioxidation capacity.PLANT CELL REPORTS,30(6),1029-1040.
MLA	Wang, Hua-Sen,et al."Overexpression in tobacco of a tomato GMPase gene improves tolerance to both low and high temperature stress by enhancing antioxidation capacity".PLANT CELL REPORTS 30.6(2011):1029-1040.