Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp pekinensis)

doi:10.1007/s11738-013-1437-0

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp pekinensis)
	Xu, Xiaoyong 1,2; Yang, Zeping 1; Sun, Xilu 1; Zhang, Lugang 1; Fang, Zhiyuan 1,3
刊名	ACTA PHYSIOLOGIAE PLANTARUM
	2014
卷号	36 期号:3 页码:809-814
关键词	Flower bud development Heading Chinese cabbage Male sterility qRT-PCR Reference gene selection
ISSN号	0137-5881
DOI	10.1007/s11738-013-1437-0
通讯作者	Xu, Xiaoyong
英文摘要	The accuracy of quantitative real-time PCR (qRT-PCR) depends on the stability of the reference gene used for normalization. In heading Chinese cabbage (Brassica rapa L. ssp. pekinensis), the most stable reference genes for qRT-PCR during flower bud development have not been elucidated. In this study, the statistical software geNorm was used to test eight candidate reference genes during flower bud development in male sterile (Ms) and fertile (Mf) plants. The result revealed that the stability order was Tub/GAPDH > Cyp > EF1a > U34559 > BrTip41 > Apr > 18S rRNA, Tub and GAPDH were the most stable genes [average expression stability (M) 0.614], and the combined use of six reference genes [pairwise variation (V) 0.15] was suggested to be the optimal reference gene for qRT-PCR during flower bud development. Furthermore, the expressions of BcPME31 during flower bud development normalized with the combined use of six reference genes and with GAPDH or Tub alone were compared; the various results also suggested that selection of the optimal reference gene was necessary for gene expression analysis.
学科主题	Plant Sciences ; PLANT SCIENCES
语种	英语
出版者	SPRINGER HEIDELBERG
WOS记录号	WOS:000331632500023
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101843]
专题	蔬菜花卉研究所
作者单位	1.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Yangling 712100, Shaanxi, Peoples R China 2.Shanxi Agr Univ, Coll Hort, Taigu 030801, Shanxi, Peoples R China 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Xu, Xiaoyong,Yang, Zeping,Sun, Xilu,et al. Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp pekinensis)[J]. ACTA PHYSIOLOGIAE PLANTARUM,2014,36(3):809-814.
APA	Xu, Xiaoyong,Yang, Zeping,Sun, Xilu,Zhang, Lugang,&Fang, Zhiyuan.(2014).Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp pekinensis).ACTA PHYSIOLOGIAE PLANTARUM,36(3),809-814.
MLA	Xu, Xiaoyong,et al."Selection of reference genes for quantitative real-time PCR during flower bud development in CMS7311 of heading Chinese cabbage (Brassica rapa L. ssp pekinensis)".ACTA PHYSIOLOGIAE PLANTARUM 36.3(2014):809-814.