Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses

doi:10.3964/j.issn.1000-0593(2016)11-3764-08

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses
	Chai Ali 1; Wang Yikai 2; Zhu Fadi 1; Shi Yanxia 1; Xie Xuewen 1; Li Baoju 1
刊名	光谱学与光谱分析
	2016
卷号	36 期号:11 页码:3764-3771
关键词	Fourier transform infrared spectroscopy (FTIR) Plant-pathogenic fungi Identification Cluster analysis Canonical vitiate analysis
ISSN号	1000-0593
DOI	10.3964/j.issn.1000-0593(2016)11-3764-08
英文摘要	Identification of plant-pathogenic fungi is time-consuming due to cultivation and microscopic examination and can be influenced by the interpretation of the micro-morphological characters observed. The present investigation aimed to create a simple but sophisticated method for the identification of plant-pathogenic fungi by Fourier transform infrared (FTIR) spectroscopy. In this study, FTIR-attenuated total reflectance (ATR) spectroscopy was used in combination with chemometric analysis for identification of important pathogenic fungi of horticultural plants. Mixtures of mycelia and spores from 27 fungal strains belonging to nine different families were collected from liquid PD or solid PDA media cultures and subjected to FTIR-ATR spectroscopy measurements. The FTIR-ATR spectra ranging from 4 000 to 400 cm~(-1) were obtained. To classify the FTIR-ATR spectra, cluster analysis was compared with canonical vitiate analysis (CVA) in the spectral regions of 3 050?2 800 and 1 800?900 cm~(-1). Results showed that the identification accuracies achieved 97.53% and 99.18% for the cluster analysis and CVA analysis, respectively, demonstrating the high potential of this technique for fungal strain identification.
学科主题	化学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101484]
专题	蔬菜花卉研究所
作者单位	1.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081 2.Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing, 100026
推荐引用方式 GB/T 7714	Chai Ali,Wang Yikai,Zhu Fadi,et al. Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses[J]. 光谱学与光谱分析,2016,36(11):3764-3771.
APA	Chai Ali,Wang Yikai,Zhu Fadi,Shi Yanxia,Xie Xuewen,&Li Baoju.(2016).Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses.光谱学与光谱分析,36(11),3764-3771.
MLA	Chai Ali,et al."Identification of Plant-Pathogenic Fungi Using Fourier Transform Infrared Spectroscopy Combined with Chemometric Analyses".光谱学与光谱分析 36.11(2016):3764-3771.