Two RAPD markers linked to a major fertility restorer gene in pepper

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Two RAPD markers linked to a major fertility restorer gene in pepper
	Zhang, BX; Huang, SW; Yang, GM; Guo, JZ
刊名	EUPHYTICA
	2000
卷号	113 期号:2 页码:155-161
关键词	bulked segregant analysis fertility restorer gene marker assisted selection (MAS) pepper (Capsicum annuum L.) random amplified polymorphic DNA (RAPD)
ISSN号	0014-2336
通讯作者	Zhang, BX
英文摘要	Both major and minor genes control the restoring of fertility in the cytoplasmic male-sterility system in pepper (Capsicum annuum L.). Bulked segregant analysis (BSA) was applied to identify molecular markers linked to a major restorer gene (Rf) using the F-2 population of Niujiaojiao No.21 (rfrf)/Xiangtanwan (RfRf). Two random amplified polymorphic DNA (RAPD) markers linked to this allele were detected with 520 decamer primers with arbitrary sequences. OP13(1400) is a tightly linked marker with a genetic distance of 0.37 cm. OW19(800) is on the opposite side with a distance of 8.12 cm. Both markers were repeatable and easy to score. A panel of genotypes, including 13 elite inbred lines with different fertility restoring ability, were assayed for the presence of OP13(1400) and OW19(800). The markers are absent in all sweet pepper lines, indicating that they will be most helpful for transferring Rf into sweet pepper lines. With the aid of these markers, the size of the backcross population for testcrosses can be minimized. Furthermore, these markers will be useful in genetic analysis of the minor genes.
学科主题	Agronomy ; Plant Sciences ; Horticulture ; AGRONOMY ; HORTICULTURE ; PLANT SCIENCES
语种	英语
出版者	KLUWER ACADEMIC PUBL
WOS记录号	WOS:000086622300009
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101265]
专题	蔬菜花卉研究所
作者单位	(1)Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
推荐引用方式 GB/T 7714	Zhang, BX,Huang, SW,Yang, GM,et al. Two RAPD markers linked to a major fertility restorer gene in pepper[J]. EUPHYTICA,2000,113(2):155-161.
APA	Zhang, BX,Huang, SW,Yang, GM,&Guo, JZ.(2000).Two RAPD markers linked to a major fertility restorer gene in pepper.EUPHYTICA,113(2),155-161.
MLA	Zhang, BX,et al."Two RAPD markers linked to a major fertility restorer gene in pepper".EUPHYTICA 113.2(2000):155-161.