A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans

doi:10.1016/j.fgb.2005.11.001

CORC > 中国农业科学院 > 蔬菜花卉研究所

	A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans
	Guo, J; Jiang, RHY; Kamphuis, LG; Govers, F
刊名	FUNGAL GENETICS AND BIOLOGY
	2006
卷号	43 期号:2 页码:111-123
关键词	cDNA-AFLP transcript profiling Phytoplithora infestans avirulence gene BSA
ISSN号	1087-1845
DOI	10.1016/j.fgb.2005.11.001
通讯作者	Guo, J
英文摘要	Expression profiling using cDNA-AFLP is commonly used to display the transcriptome of a specific tissue or developmental stage. Here, cDNA-AFLP was used to identify transcripts in a segregating F1 population of Phytophthora infestans, the oomycete pathogen that causes late blight. To find transcripts derived from putative avirulence (Avr) genes germinated cyst cDNA from F1 progeny with defined avirulence phenotypes was pooled and used in a bulked segregant analysis (BSA). Over 30,000 transcript derived fragments (TDFs) were screened resulting in 99 Avr-associated TDFs as well as TDFs with opposite pattern. With 142 TDF sequences homology searches and database mining was carried out. cDNA-AFLP analysis on individual F1 progeny revealed 100% co-segregation of four TDFs with particular AVR phenotypes and this was confirmed by RT-PCR. Two match the same P. infestans EST with unknown sequence and this is a likely candidate for Avr4. The other two are associated with the Aur3b-Avr10-Avr11 locus. This combined cDNA-AFLP/BSA strategy is an efficient approach to identify Aur-associated transcriptome markers that can complement positional cloning. (c) 2005 Elsevier Inc. All rights reserved.
学科主题	Genetics & Heredity ; Mycology ; GENETICS & HEREDITY ; MYCOLOGY
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
WOS记录号	WOS:000235779400005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101246]
专题	蔬菜花卉研究所
作者单位	(1)Univ Wageningen, Phytopathol Lab, Plant Sci Grp, NL-6709 PD Wageningen, Netherlands;(2)Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China;(3)NW A & F Univ, Coll Plant Protect, Yangling 712100, Shaanxi, Peoples R China
推荐引用方式 GB/T 7714	Guo, J,Jiang, RHY,Kamphuis, LG,et al. A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans[J]. FUNGAL GENETICS AND BIOLOGY,2006,43(2):111-123.
APA	Guo, J,Jiang, RHY,Kamphuis, LG,&Govers, F.(2006).A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans.FUNGAL GENETICS AND BIOLOGY,43(2),111-123.
MLA	Guo, J,et al."A cDNA-AFLP based strategy to identify transcripts associated with avirulence in Phytophthora infestans".FUNGAL GENETICS AND BIOLOGY 43.2(2006):111-123.