The Phytophthora infestans Avirulence Gene Avr4 Encodes an RXLR- dEER Effector

doi:10.1094/MPMI-21-11-1460

CORC > 中国农业科学院 > 蔬菜花卉研究所

	The Phytophthora infestans Avirulence Gene Avr4 Encodes an RXLR- dEER Effector
	van Poppel, Pieter M. J. A.1,2; Guo, Jun 1,3,4; de Vondervoort, Peter J. I. van 1; Jung, Maartje W. M.1; Birch, Paul R. J.5; Whisson, Stephen C.5; Govers, Francine 1,2
刊名	MOLECULAR PLANT-MICROBE INTERACTIONS
	2008
卷号	21 期号:11 页码:1460-1470
关键词	late blight plant pathogen RXLR motif
ISSN号	0894-0282
DOI	10.1094/MPMI-21-11-1460
通讯作者	van Poppel, Pieter M. J. A.
英文摘要	Resistance in potato against the oomycete Phytophthora infestans is conditioned by resistance (R) genes that are introgressed from wild Solanum spp. into cultivated potato. According to the gene-for-gene model, proteins encoded by R genes recognize race-specific effectors resulting in a hypersensitive response (HR). We isolated P. infestans avirulence gene PiAvr4 using a combined approach of genetic mapping, transcriptional profiling, and bacterial artificial chromosome marker landing. PiAvr4 encodes a 287-amino-acidprotein that belongs to a superfamily of effectors sharing the putative host-cell-targeting motif RXLR-dEER. Transformation of P. infestans race 4 strains with PiAvr4 resulted in transformants that were avirulent on R4 potato plants, demonstrating that PiAvr4 is responsible for eliciting R4-mediated resistance. Moreover, expression of PiAvr4 in R4 plants using PVX agroinfection and agroinfiltration showed that PiAvr4 itself is the effector that elicits HR on R4 but not r0 plants. The presence of the RXLR-dEER motif suggested intracellular recognition of PiAvr4. This was confirmed in agroinfiltration assays but not with PVX agroinfection. Because there was always recognition of PiAvr4 retaining the signal peptide, extracellular recognition cannot be excluded. Deletion of the RXLR-dEER domain neither stimulated nor prevented elicitor activity of PiAvr4. Race 4 strains have frame shift mutations in PiAvr4 that result in truncated peptides; hence, PiAvr4 is apparently not crucial for virulence.
学科主题	Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Plant Sciences ; BIOCHEMISTRY & MOLECULAR BIOLOGY ; BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; PLANT SCIENCES
语种	英语
出版者	AMER PHYTOPATHOLOGICAL SOC
WOS记录号	WOS:000260226500007
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101226]
专题	蔬菜花卉研究所
作者单位	1.Univ Wageningen & Res Ctr, Phytopathol Lab, NL-6709 PD Wageningen, Netherlands 2.CBSG, NL-6700 AB Wageningen, Netherlands 3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 4.NW A&F Univ, Coll Plant Protect, Yangling Shaanxi 712100, Peoples R China 5.Scottish Crop Res Inst, Plant Pathol Programme, Dundee DD2 5DA, Scotland
推荐引用方式 GB/T 7714	van Poppel, Pieter M. J. A.,Guo, Jun,de Vondervoort, Peter J. I. van,et al. The Phytophthora infestans Avirulence Gene Avr4 Encodes an RXLR- dEER Effector[J]. MOLECULAR PLANT-MICROBE INTERACTIONS,2008,21(11):1460-1470.
APA	van Poppel, Pieter M. J. A..,Guo, Jun.,de Vondervoort, Peter J. I. van.,Jung, Maartje W. M..,Birch, Paul R. J..,...&Govers, Francine.(2008).The Phytophthora infestans Avirulence Gene Avr4 Encodes an RXLR- dEER Effector.MOLECULAR PLANT-MICROBE INTERACTIONS,21(11),1460-1470.
MLA	van Poppel, Pieter M. J. A.,et al."The Phytophthora infestans Avirulence Gene Avr4 Encodes an RXLR- dEER Effector".MOLECULAR PLANT-MICROBE INTERACTIONS 21.11(2008):1460-1470.