Population Dynamics of Phytophthora infestans in the Netherlands Reveals Expansion and Spread of Dominant Clonal Lineages and Virulence in Sexual Offspring

doi:10.1534/g3.112.004150

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Population Dynamics of Phytophthora infestans in the Netherlands Reveals Expansion and Spread of Dominant Clonal Lineages and Virulence in Sexual Offspring
	Li, Y.1,2; van der Lee, T. A. J.1; Evenhuis, A.1; van den Bosch, G. B. M.1; van Bekkum, P. J.1; Forch, M. G.1; van Gent-Pelzer, M. P. E.1; van Raaij, H. M. G.1; Jacobsen, E.3; Huang, S. W.2
刊名	G3-GENES GENOMES GENETICS
	2012
卷号	2 期号:12 页码:1529-1540
关键词	late blight Blue_13 avirulence microsatellites population genetics
ISSN号	2160-1836
DOI	10.1534/g3.112.004150
通讯作者	Li, Y.
英文摘要	For a comprehensive survey of the structure and dynamics of the Dutch Phytophthora infestans population, 652 P. infestans isolates were collected from commercial potato fields in the Netherlands during the 10-year period 2000-2009. Genotyping was performed using 12 highly informative microsatellite markers and mitochondrial haplotypes. In addition, for each isolate, the mating type was determined. STRUCTURE analysis grouped the 322 identified genotypes in three clusters. Cluster 1 consists of a single clonal lineage NL-001, known as "Blue_13"; all isolates in this cluster have the A2 mating type and the Ia mitochondrial haplotype. Clusters 2 and 3 display a more elaborate substructure containing many unique genotypes. In Cluster 3, several distinct clonal lineages were also identified. This survey witnesses that the Dutch population underwent dramatic changes in the 10 years under study. The most notable change was the emergence and spread of A2 mating type strain NL-001 (or "Blue_13"). The results emphasize the importance of the sexual cycle in generating genetic diversity and the importance of the asexual cycle as the propagation and dispersal mechanism for successful genotypes. Isolates were also screened for absence of the Avrblb1/ipiO class I gene, which is indicative for virulence on Rpi-blb1. This is also the first report of Rpi-blb1 breakers in the Netherlands. Superimposing the virulence screening on the SSR genetic backbone indicates that lack the Avrblb1/ipiO class I gene only occurred in sexual progeny. So far, the asexual spread of the virulent isolates identified has been limited.
学科主题	Genetics & Heredity ; GENETICS & HEREDITY
语种	英语
出版者	GENETICS SOC AM
WOS记录号	WOS:000312458000005
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/101080]
专题	蔬菜花卉研究所
作者单位	1.Plant Res Int, Biointeract & Plant Hlth, NL-6700 AA Wageningen, Netherlands 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Wageningen Univ, Lab Plant Breeding, NL-6708 PB Wageningen, Netherlands 4.Wageningen Univ, Phytopathol Lab, NL-6700 EE Wageningen, Netherlands 5.Ctr BioSyst Biol, NL-6700 AB Wageningen, Netherlands
推荐引用方式 GB/T 7714	Li, Y.,van der Lee, T. A. J.,Evenhuis, A.,et al. Population Dynamics of Phytophthora infestans in the Netherlands Reveals Expansion and Spread of Dominant Clonal Lineages and Virulence in Sexual Offspring[J]. G3-GENES GENOMES GENETICS,2012,2(12):1529-1540.
APA	Li, Y..,van der Lee, T. A. J..,Evenhuis, A..,van den Bosch, G. B. M..,van Bekkum, P. J..,...&Kessel, G. J. T..(2012).Population Dynamics of Phytophthora infestans in the Netherlands Reveals Expansion and Spread of Dominant Clonal Lineages and Virulence in Sexual Offspring.G3-GENES GENOMES GENETICS,2(12),1529-1540.
MLA	Li, Y.,et al."Population Dynamics of Phytophthora infestans in the Netherlands Reveals Expansion and Spread of Dominant Clonal Lineages and Virulence in Sexual Offspring".G3-GENES GENOMES GENETICS 2.12(2012):1529-1540.