Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth

CORC > 中国农业科学院 > 蔬菜花卉研究所

	Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth
	Yi Dengxia; Cui Lei; Liu Yumei; Zhuang Mu; Zhang Yangyong; Fang Zhiyuan; Yang Limei
刊名	Agricultural Sciences in China
	2011
卷号	10 期号:11 页码:1693-1700
关键词	Bacillus thuringiensis cry1Ba3 cabbage diamondback moth resistance
ISSN号	1671-2927
英文摘要	To obtain transgenic cabbage line with broad insect resistance, a new synthetic Bacillus thuringiensis cry1Ba3 gene was introduced into white cabbage via Agrobacterium tumefaciens-mediated transformation and 37 transformants were obtained. Polymerase chain reaction (PCR) and Southern blot analyses confirmed that cry1Ba3 was successfully inserted into the genome of cabbage. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that cry1Ba3 was expressed. Western blot results confirmed the production of insecticidal protein encoded by cry1Ba3. Insect bioassays showed that transgenic cabbages effectively controlled both susceptible and Cry1Ac-resistant diamondback moth (DBM) larvae.
学科主题	植物保护
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100625]
专题	蔬菜花卉研究所
作者单位	Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Horticultural Crops Genetic Improvement, Ministry of Agriculture, Beijing, 100081
推荐引用方式 GB/T 7714	Yi Dengxia,Cui Lei,Liu Yumei,et al. Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth[J]. Agricultural Sciences in China,2011,10(11):1693-1700.
APA	Yi Dengxia.,Cui Lei.,Liu Yumei.,Zhuang Mu.,Zhang Yangyong.,...&Yang Limei.(2011).Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth.Agricultural Sciences in China,10(11),1693-1700.
MLA	Yi Dengxia,et al."Transformation of Cabbage (Brassica oleracea L. var. capitata) with Bt cry1Ba3 Gene for Control of Diamondback Moth".Agricultural Sciences in China 10.11(2011):1693-1700.