Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence

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	Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence
	Lin Ling-ling 2; Shi Qing-hua 2; Wang Hua-sen 3; Qin Ai-guo 2; Yu Xian-chang 1
刊名	Agricultural Sciences in China
	2011
卷号	10 期号:4 页码:534-543
关键词	potato GDP-mannose pyrophosphorylase ascorbate senescence
ISSN号	1671-2927
英文摘要	GDP-D-mannose pyrophosphorylase (GMPase) catalyses the synthesis of GDP-D-mannose and represents the first committed step in the synthesis of ascorbate. In the present study, the GMPase gene of tomato was introduced into potato by Agrobacterium-mediated transformation. Two transgenic lines with higher GMPase expression were selected using qPCR and protein blot analyses. The results showed that the content of L-ascorbic acid (AsA) and the ratio of AsA/ DHA (dehydroascorbate) significantly increased in both leaves and tubers of transgenic potato plants. Both pigment content and photosynthetic rate were much higher in transgenic plants than in wild-type plants. Transgenic plants showed a distinguishable change in phenotype from the wild-type plants. Furthermore, transgenic plants showed delayed senescence.
学科主题	农作物
语种	英语
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100623]
专题	蔬菜花卉研究所
作者单位	1.Shandong Agr Univ, Coll Hort Sci & Engn, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China 2.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China 3.Zhejiang Forestry Univ, Coll Agr & Food Sci, Linan 311300, Peoples R China
推荐引用方式 GB/T 7714	Lin Ling-ling,Shi Qing-hua,Wang Hua-sen,et al. Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence[J]. Agricultural Sciences in China,2011,10(4):534-543.
APA	Lin Ling-ling,Shi Qing-hua,Wang Hua-sen,Qin Ai-guo,&Yu Xian-chang.(2011).Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence.Agricultural Sciences in China,10(4),534-543.
MLA	Lin Ling-ling,et al."Over-Expression of Tomato GDP-Mannose Pyrophosphorylase (GMPase) in Potato Increases Ascorbate Content and Delays Plant Senescence".Agricultural Sciences in China 10.4(2011):534-543.