An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber

doi:10.1111/jipb.12355

CORC > 中国农业科学院 > 蔬菜花卉研究所

	An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber
	Zhou Qian 1; Wang Shenhao 1; Hu Bowen 1; Chen Huiming 2; Zhang Zhonghua 1; Huang Sanwen 1
刊名	Journal of Integrative Plant Biology
	2015
卷号	57 期号:11 页码:936-942
关键词	ARC5 Cucumis sativus ethyl methane sulfonate mutant fruit peel color whole genome re-sequencing
ISSN号	1672-9072
DOI	10.1111/jipb.12355
英文摘要	The peel color of fruit is an important commercial trait in cucumber, but the underlying molecular basis is largely unknown. A mutant showing light green exocarp was discovered from ethyl methane sulfonate (EMS) mutagenized cucumber line 406 with dark green exocarp. Genetic analysis showed the mutant phenotype is conferred by a single recessive gene, here designated as Igp (light green peel). By re-sequencing of bulked segregants, we identified the candidate gene Csa7G051430 encoding ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 (ARC5) that plays a vital role in chloroplast division in Arabidopsis. A single nucleotide polymorphism (SNP) causing amino acid alteration in the conserved GTPase domain of Csa7G05i430 showed co-segregation with the altered phenotype. Furthermore, the transient RNA interference of this gene resulted in reduced number and enlarged size of chloroplasts, which were also observed in the Igp mutant. This evidence supports that the non-synonymous SNP in Csa7Go51430 is the causative mutation for the light green peel. This study provides a new allele for cucumber breeding for light green fruits and additional resource for the study of chloroplast development.
学科主题	细胞生物学 ; 分子生物学
语种	中文
内容类型	期刊论文
源URL	[http://111.203.20.206/handle/2HMLN22E/100566]
专题	蔬菜花卉研究所
作者单位	1.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Beijing, 100081 2.Hunan Vegetable Research Institute, Hunan Academy of Agricultural Science, Changsha, 410125
推荐引用方式 GB/T 7714	Zhou Qian,Wang Shenhao,Hu Bowen,et al. An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber[J]. Journal of Integrative Plant Biology,2015,57(11):936-942.
APA	Zhou Qian,Wang Shenhao,Hu Bowen,Chen Huiming,Zhang Zhonghua,&Huang Sanwen.(2015).An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber.Journal of Integrative Plant Biology,57(11),936-942.
MLA	Zhou Qian,et al."An ACCUMULATION AND REPLICATION OF CHLOROPLASTS 5 gene mutation confers light green peel in cucumber".Journal of Integrative Plant Biology 57.11(2015):936-942.