Hydrophobic pocket modification and humanized catalytic antibodies with glutathione peroxidase activity (I) - Preparation and characterization of haptenes and conjugates

	Hydrophobic pocket modification and humanized catalytic antibodies with glutathione peroxidase activity (I) - Preparation and characterization of haptenes and conjugates
	Wang L ; Ma XY ; Ding L ; Zhao DQ ; Ni JZ ; Fu PP ; Li XP ; Jin XJ
刊名	chemical research in chinese universities
	1998
卷号	14 期号:4 页码:414-419
ISSN号	1005-9040
中文摘要	the thiol group of glutathione (gsh) was protected by 2,4-dinitrochlorobenzene (dncb), the product s-substituted dinitrophenyl gsh(gsh-s-dnp) was alcoholized to obtain haptenes 4 and 5 respectively. as haptenes, the two gsh derivatives were characterized by means of h-1 nmr, maldi-tof-ms and ir, followed by individually coupling with bovine serum albumin (bsa) via glutaraldehyde. bsb-hp4 and bsa-hp5 were purified by sephadex g-25 gel filtration chromatography. for each conjugate, the average haptene-bsa ratio was 12 : 1. the electrophoresis analysis showed that the average molecular weight of each conjugate was 76 500. the cd spectrum indicated that the conjugates had more a-helix content than bsa did.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000078261200014
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/22577]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang L,Ma XY,Ding L,et al. Hydrophobic pocket modification and humanized catalytic antibodies with glutathione peroxidase activity (I) - Preparation and characterization of haptenes and conjugates[J]. chemical research in chinese universities,1998,14(4):414-419.
APA	Wang L.,Ma XY.,Ding L.,Zhao DQ.,Ni JZ.,...&Jin XJ.(1998).Hydrophobic pocket modification and humanized catalytic antibodies with glutathione peroxidase activity (I) - Preparation and characterization of haptenes and conjugates.chemical research in chinese universities,14(4),414-419.
MLA	Wang L,et al."Hydrophobic pocket modification and humanized catalytic antibodies with glutathione peroxidase activity (I) - Preparation and characterization of haptenes and conjugates".chemical research in chinese universities 14.4(1998):414-419.