Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom

	Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom
	Sun MZ ; Ding L ; Ji YP ; Zhao DQ ; Liu SY ; Ni JZ
刊名	rapid communications in mass spectrometry
	1999
卷号	13 期号:3 页码:150-155
关键词	WESTERN DIAMONDBACK RATTLESNAKE SIZE-EXCLUSION CHROMATOGRAPHY CONTORTRIX-CONTORTRIX CROTALUS-ATROX DESORPTION SNAKE RESOLUTION ACID
ISSN号	0951-4198
通讯作者	zhao, dq, chinese acad sci, changchun inst appl chem, key lab rare earth chem & phys, changchun 130022, peoples r china
中文摘要	matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tofms) was used to analyze two enzymes, phospholipase az and fibrinolytic enzyme isolated from chinese agkistrodon blomhoffii ussurensis venom. using sinapinic acid as the matrix, positive ion mass spectra of the enzymes were obtained, in addition to the dominant protein [m+h](+) ions, multimeric and multiply charged ions were also observed in the mass spectra, the higher the concentration of the enzymes, the more multiply charged polymer and multimeric ions were detected, our results indicate that maldi-tofms can provide a rapid and accurate method for molecular weight determination of snake venom enzymes, mass accuracies of 0.1 and 0.3 % were achieved by analysis of highly dialyzed phospholipase a2 and fibrinolytic enzyme, and these results are much better than those obtained using sodium dodecyl sulfate-palyacrylamide gel electrophoresis. maldi-tofms thus provides a reliable method to determine the purity and molecular weight of these enzymes, which are of potential use as therapeutants, copyright (c) 1999 john wiley & sons, ltd.
收录类别	CSCD收录国内期刊论文
语种	英语
WOS记录号	WOS:000078534100002
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/22027]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Sun MZ,Ding L,Ji YP,et al. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom[J]. rapid communications in mass spectrometry,1999,13(3):150-155.
APA	Sun MZ,Ding L,Ji YP,Zhao DQ,Liu SY,&Ni JZ.(1999).Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom.rapid communications in mass spectrometry,13(3),150-155.
MLA	Sun MZ,et al."Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric analysis of phospholipase A(2) and fibrinolytic enzyme, two enzymes obtained from Chinese Agkistrodon blomhoffii Ussurensis venom".rapid communications in mass spectrometry 13.3(1999):150-155.