Fluorescence spectra of hemorrhagin III from the snake venom of Agkistrodon acutus

	Fluorescence spectra of hemorrhagin III from the snake venom of Agkistrodon acutus
	Chen ZL ; Zhao DQ ; Liu QL ; Ding L ; Zhang ZD ; Ni JZ
刊名	chinese journal of inorganic chemistry
	1999
卷号	15 期号:2 页码:185-190
关键词	PURIFICATION PROTEINASE
ISSN号	1001-4861
通讯作者	chen zl
中文摘要	hemorrhagin iii (aah iii) was separated and purified from the crude snake venom of agkistrodon acutus, and its molecule weight was determined accurately to be 23; 284.4 +/- 0.1 by ldi1700-maldi-tof-ms. emission spectra of aah iii showed that trp residues were located by a great degree in the hydrophobic area. addition of sds and guanidine-hcl led to change of the molecular conformation of aah iii, and caused the fluorescence quenching of trp residues. the red-shifted emission band of aah iii after adding guanidine-hcl showed that trp residues exposed in polar solvents. the effects of ph, edta and metal ions on the fluorescence spectra of aah iii were also investigated.
收录类别	SCI收录期刊论文
语种	中文
WOS记录号	WOS:000084247500007
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/21477]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Chen ZL,Zhao DQ,Liu QL,et al. Fluorescence spectra of hemorrhagin III from the snake venom of Agkistrodon acutus[J]. chinese journal of inorganic chemistry,1999,15(2):185-190.
APA	Chen ZL,Zhao DQ,Liu QL,Ding L,Zhang ZD,&Ni JZ.(1999).Fluorescence spectra of hemorrhagin III from the snake venom of Agkistrodon acutus.chinese journal of inorganic chemistry,15(2),185-190.
MLA	Chen ZL,et al."Fluorescence spectra of hemorrhagin III from the snake venom of Agkistrodon acutus".chinese journal of inorganic chemistry 15.2(1999):185-190.