The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4)(2)SO4 aqueous two-phase system

	The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4)(2)SO4 aqueous two-phase system
	Xu Y ; Lu J ; Li DQ
刊名	preparative biochemistry & biotechnology
	2001
卷号	31 期号:1 页码:23-31
关键词	2-PHASE SYSTEMS SEPARATION
ISSN号	1082-6068
通讯作者	li dq
中文摘要	the effect of potassium thiocyanate on the partitioning of lysozyme and bsa in polyethylene glycol 2000/ammonium sulfate aqueous two-phase system has been investigated. as a result of the addition of potassium thiocyanate to the peg/ammonium sulfate system, the peg/mixed salts aqueous two-phase system was formed. it was found that the potassium thiocyanate could alter the ph difference between the two phases, and, thus, influence the partition coefficients of the differently charged proteins. the relationship between partition coefficient of the proteins and ph difference between two phases has been discussed. it was proposed that the ph difference between two phases could be employed as the measurement of electrostatic driving force for the partitioning of charged proteins in polyethylene glycol 2000/ammonium sulfate aqueous two-phase system.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000169923800003
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/21429]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Xu Y,Lu J,Li DQ. The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4)(2)SO4 aqueous two-phase system[J]. preparative biochemistry & biotechnology,2001,31(1):23-31.
APA	Xu Y,Lu J,&Li DQ.(2001).The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4)(2)SO4 aqueous two-phase system.preparative biochemistry & biotechnology,31(1),23-31.
MLA	Xu Y,et al."The effect of KSCN on the partition of proteins in polyethylene glycol/(NH4)(2)SO4 aqueous two-phase system".preparative biochemistry & biotechnology 31.1(2001):23-31.