Self-assembled monolayer growth of phospholipids on hydrophobic surface toward mimetic biomembranes: Scanning probe microscopy study

	Self-assembled monolayer growth of phospholipids on hydrophobic surface toward mimetic biomembranes: Scanning probe microscopy study
	Tang ZY ; Jing WG ; Wang EK
刊名	langmuir
	2000
卷号	16 期号:4 页码:1696-1702
关键词	ATOMIC-FORCE MICROSCOPY LANGMUIR-BLODGETT-FILMS AIR-WATER-INTERFACE QUARTZ-CRYSTAL MICROBALANCE TUNNELING-MICROSCOPY MOLECULAR-RESOLUTION LIPID BILAYER PHOSPHATIDYLCHOLINE BILAYERS INTERDIGITATED DOMAINS CHOLERA-TOXIN
ISSN号	0743-7463
通讯作者	wang ek
中文摘要	atomic force microscopy (afm) and lateral force microscopy (lfm) were used simultaneously to analyze a model membrane bilayer structure consisting of a phospholipid outer monolayer deposited onto organosilane-derivatized mica surfaces, which were constructed by using painting and self-assembly methods. the phospholipid used as outer monolayer was dimyristoylphosphatidylcholine (dmpc). the hydrocarbon-covered substrate that formed the inner half bilayer was composed of a self-assembly monolayer (sam) of octadecyltrichloroorganosilane (ots) on mica. sams of dmpc were formed by exposing hydrophobic mica to a solution of dmpc in decane/isobutanol and subsequently immersing into pure water. afm images of samples immersed in solution for varying exposure times showed that before forming a complete monolayer the molecules aggregated into dense islands (2.2-2.6 nm high) on the surface. the islands had a compact and rounded morphology. lfm, coupled with topographic data obtained with the atomic force mode, had made possible the distinction between dmpc and ots. the rate constant of dmpc growth was calculated. this is the first systematic study of the sam formation of dmpc by afm and lfm imaging. it reveals more direct information about the film morphology than previous studies with conventional surface analytical techniques such as infrared spectroscopy, x-ray, or fluorescence microscopy.
收录类别	SCI收录期刊论文
语种	英语
WOS记录号	WOS:000085412600033
公开日期	2010-11-04
内容类型	期刊论文
源URL	[http://202.98.16.49/handle/322003/19891]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Tang ZY,Jing WG,Wang EK. Self-assembled monolayer growth of phospholipids on hydrophobic surface toward mimetic biomembranes: Scanning probe microscopy study[J]. langmuir,2000,16(4):1696-1702.
APA	Tang ZY,Jing WG,&Wang EK.(2000).Self-assembled monolayer growth of phospholipids on hydrophobic surface toward mimetic biomembranes: Scanning probe microscopy study.langmuir,16(4),1696-1702.
MLA	Tang ZY,et al."Self-assembled monolayer growth of phospholipids on hydrophobic surface toward mimetic biomembranes: Scanning probe microscopy study".langmuir 16.4(2000):1696-1702.