Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions

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	Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions
	Long M(龙勉); Zhao H; Huang KS
刊名	Annals of Biomedical Engineering
	2001
卷号	29 期号:11 页码:935-946
ISSN号	0090-6964
通讯作者	Zhu, C (reprint author), Georgia Inst Technol, George W Woodruff Sch Mech Engn, Atlanta, GA 30332 USA.
中文摘要	Selectin/ligand interactions initiate the multistep adhesion and signaling cascades in the recruitment of leukocytes from circulation to inflamed tissues and may also play a role in tumor metastasis. Kinetic properties of these interactions are essential determinants governing blood-borne cells' tethering to and rolling on the vessel wall. Extending our recently developed micropipette method, we have measured the kinetic rates of E-selectin/ligand interactions. Red cells coated with an E-selectin construct were allowed to bind HL-60 or Colo-205 cells bearing carbohydrate ligands. Specific adhesions were observed to occur at isolated points, the frequency of which followed a Poisson distribution. These point attachments were formed at the same rate with both the HL-60 and Colo-205 cells (0.14 +/- 0.04 and 0.13 +/- 0.03 mum(2) s(-1) per unit density of E-selectin, respectively) but dissociated from the former at a rate twice as fast as did from the latter (0.92 +/- 0.23 and 0.44 +/- 0.10 s(-1), respectively). The reverse rates agree well with those measured by the flow chamber. The forward rates are orders of magnitude higher than those of Fc gamma receptors interacting with IgG measured under similar conditions, consistent with the rapid kinetics requirement for the function of E-selectin/ligand binding, which is to capture leukocytes on endothelial surfaces from flow.
学科主题	力学
类目[WOS]	Engineering, Biomedical
研究领域[WOS]	Engineering
关键词[WOS]	SELECTIN GLYCOPROTEIN LIGAND-1 ; P-SELECTIN ; CARBOHYDRATE INTERACTIONS ; ADHESION MOLECULES ; CONCURRENT BINDING ; HUMAN-LYMPHOCYTES ; HUMAN NEUTROPHILS ; ASSOCIATION RATE ; GROWTH-FACTOR ; CANCER-CELLS
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000172760500002
公开日期	2007-06-15
内容类型	期刊论文
源URL	[http://dspace.imech.ac.cn/handle/311007/16891]
专题	力学研究所_力学所知识产出(1956-2008)
推荐引用方式 GB/T 7714	Long M,Zhao H,Huang KS. Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions[J]. Annals of Biomedical Engineering,2001,29(11):935-946.
APA	Long M,Zhao H,&Huang KS.(2001).Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions.Annals of Biomedical Engineering,29(11),935-946.
MLA	Long M,et al."Kinetic measurements of cell surface E-selectin/carbohydrate ligand interactions".Annals of Biomedical Engineering 29.11(2001):935-946.