Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity

	Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity
	Wang J ; Hong A ; Ren JS ; Sun FY ; Shi YJ ; Liu K ; Xie QL ; Dai Y ; Li ZY ; Chen Y
刊名	journal of biotechnology
	2006
卷号	121 期号:4 页码:442-447
关键词	FIBROBLAST-GROWTH-FACTOR SELF-ASSOCIATION HEPARIN FACTOR-2 BINDING
ISSN号	0168-1656
通讯作者	wang j
中文摘要	fibroblast growth factor-2 (fgf-2) is a multifunctional polypeptide that affects many cellular functions and phenomena. the wild-type recombinant human fibroblast growth factor rhfgf-2(w) and the mutant c78sc96s rhfgf-2(m) were expressed in escherichia coli and their products were purified. the results by the means of fluorescence spectroscopy and cd spectrums, suggested that due to its decreased hydrophobicity rhfgf-2 is not deposited as an inclusion body. the mitogenic activity of the expressed rhfgf-2(m) on 3t3 fibroblasts was shown to be 10-fold more than the expressed rhfgf-2(w) of which the biological activity was a little less than that of the standard rhbfgf(w), indicating that the increased biological activity was due to the change of its secondary structure, dimerization and affinity binding to fgf receptor (fgfr).
收录类别	SCI
语种	英语
WOS记录号	WOS:000235697100002
公开日期	2010-08-17
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/16261]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Wang J,Hong A,Ren JS,et al. Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity[J]. journal of biotechnology,2006,121(4):442-447.
APA	Wang J.,Hong A.,Ren JS.,Sun FY.,Shi YJ.,...&Chen Y.(2006).Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity.journal of biotechnology,121(4),442-447.
MLA	Wang J,et al."Biochemical properties of C78SC96S rhFGF-2: A double point-mutated rhFGF-2 increases obviously its activity".journal of biotechnology 121.4(2006):442-447.