Novel prefractionation method can be used in proteomic analysis

	Novel prefractionation method can be used in proteomic analysis
	Bai HX ; Yang F ; Yang XR
刊名	journal of proteome research
	2006
卷号	5 期号:4 页码:840-845
关键词	AQUEOUS 2-PHASE SYSTEMS PROTEIN-PURIFICATION MEMBRANE-PROTEINS EXTRACTION FRACTIONATION EQUILIBRIUM COPOLYMER
ISSN号	1535-3893
通讯作者	yang xr
中文摘要	for the first time, a novel prefractionation method used in proteomic analysis was developed, which is performed by a novel aqueous two-phase system (natps) composed of n-butanol, (nh4)(2)so4, and water. it can separate proteomic proteins into multigroups by one-step extraction. the phase-separation conditions of n-butanol solutions were studied in the presence of commonly used inorganic salts. the natps was subsequently developed. using human serum albumin, zein, and gamma-globulin as model proteins, the separation effectiveness of the natps for protein was studied under affection factors, i.e., ph, n-butanol volume, protein, or salt concentration. the model and actual protein samples were separated by the natps and then directly used for gel electrophoresis without separating the target proteins from phase-forming reagents. it revealed that the natps could separate proteomic proteins into multigroups by one-step extraction. the natps has the advantages of rapidity, simplicity, low cost, biocompability, and high efficiency. it need not separate target proteins from the phase-forming reagents. the natps has great significance in separation and extraction of proteomic proteins, as well as in methodology.
收录类别	SCI
语种	英语
WOS记录号	WOS:000236816100013
公开日期	2010-08-17
内容类型	期刊论文
源URL	[http://ir.ciac.jl.cn/handle/322003/15857]
专题	长春应用化学研究所_长春应用化学研究所知识产出_期刊论文
推荐引用方式 GB/T 7714	Bai HX,Yang F,Yang XR. Novel prefractionation method can be used in proteomic analysis[J]. journal of proteome research,2006,5(4):840-845.
APA	Bai HX,Yang F,&Yang XR.(2006).Novel prefractionation method can be used in proteomic analysis.journal of proteome research,5(4),840-845.
MLA	Bai HX,et al."Novel prefractionation method can be used in proteomic analysis".journal of proteome research 5.4(2006):840-845.