Sequence Analysis of the Coat Protein Gene of  Bean yellow mosaic virus Isolates from  Faba bean in Yunnan, China and Syria

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	Sequence Analysis of the Coat Protein Gene of Bean yellow mosaic virus Isolates from Faba bean in Yunnan, China and Syria
	QI Peng 1; WANG Xiaoming 1; HE Yueqiu 2
刊名	植物病理学报
	2007
卷号	37 期号:4 页码:368-376
关键词	Bean yellow mosaic virus faba bean isolate coat protein sequence analysis
ISSN号	0412-0914
其他题名	菜豆黄花叶病毒中国和叙利亚蚕豆分离物外壳蛋白的序列分析
英文摘要	Viral diseases are one of the major factors for yield loss of faba bean in Yunnan province, China. Bean yellow mosaic virus （BYMV） was identified in faba bean samples by Tissue Blot Immunoassay （TBIA）. According to BYMV sequence submitted to GenBank, a pair of primer was designed and used to amplify the coat protein （CP） gene of BYMV in six faba bean isolates, five from Yunnan province and one from Syria, by reverse transcription PCR （RT-PCR）. The amplified fragment was cloned and in which 822 nucleotides was for CP gene. The CP gene was found to be 86.4% to 100.0% and 96.7% to 100.0% homologous among six BYMV isolates at both the nucleotide and amino acid level. The CP gene of six sequenced isolates showed 79.1% to 97.9% and 83.5% to 98.5% nucleotide and amino acid identifies with 34 BYMV isolates in GenBank. The comparison data of CP gene sequences showed that BYMV-Chinese faba bean isolates were closely related to the BYMV-Japanese faba bean isolates. The NAG motif, a basic structure for aphid transmission of the virus located at the N-terminus of CP gene in BYMV, was presented in all of six isolates.
学科主题	植物保护
语种	英语
内容类型	期刊论文
源URL	[http://ir.nais.net.cn/handle/2HMLN22E/131376]
专题	作物科学研究所
作者单位	1.The National Key Facility for Crop Gene Resources and Genetic Improvement, NFCRI, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081; 2.Plant Protection College, Yunnan Agriculture University, Kanming, 650201
推荐引用方式 GB/T 7714	QI Peng,WANG Xiaoming,HE Yueqiu. Sequence Analysis of the Coat Protein Gene of Bean yellow mosaic virus Isolates from Faba bean in Yunnan, China and Syria[J]. 植物病理学报,2007,37(4):368-376.
APA	QI Peng,WANG Xiaoming,&HE Yueqiu.(2007).Sequence Analysis of the Coat Protein Gene of Bean yellow mosaic virus Isolates from Faba bean in Yunnan, China and Syria.植物病理学报,37(4),368-376.
MLA	QI Peng,et al."Sequence Analysis of the Coat Protein Gene of Bean yellow mosaic virus Isolates from Faba bean in Yunnan, China and Syria".植物病理学报 37.4(2007):368-376.